UM
Nanoporous membrane-encapsulated feeder cells for culture of human embryonic stem cells
Jing Zhou1; Ren-He Xu2; Yong Wang3
2009
Source PublicationInternational Journal of Functional Informatics and Personalised Medicine
ISSN17562112 17562104
Volume2Issue:1Pages:77-88
Abstract

Current methods for the extended culture of human embryonic stem cells (hESCs) are far from optimal. Therefore, we proposed to use nanoporous membrane for the co-culture of feeder cells and hESCs. In this proof-of-concept study, we first studied the stability, biocompatibility and permeability of an alginate-poly-L-lysine-alginate membrane. This membrane was then used to encapsulate feeder cells to examine the cell viability and TGF-ß secretion. Finally, the encapsulated feeder-conditioned media were used to culture hESCs. The data indicate that nanoporous membrane is a potential tool for the co-culture of feeder cells and hESCs in a safe, efficient, and continuous manner. © 2009 Inderscience Enterprises Ltd.

KeywordHescs Human Embryonic Stem Cells Mef Mouse Embryonic Fibroblasts Nanoporous Membrane Pathogen Self-renewal
DOI10.1504/IJFIPM.2009.022838
URLView the original
Fulltext Access
Citation statistics
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Department of Chemical, Materials and Biomolecular Engineering, University of Connecticut, Storrs, CT 06269, USA
2.UConn Stem Cell Institute, Department of Genetics and Developmental Biology, University of Connecticut Health Centre, Farmington, CT 06032, USA
3.Department of Chemical, Materials and Biomolecular Engineering, University of Connecticut, Storrs, CT 06269, USA
Recommended Citation
GB/T 7714
Jing Zhou,Ren-He Xu,Yong Wang. Nanoporous membrane-encapsulated feeder cells for culture of human embryonic stem cells[J]. International Journal of Functional Informatics and Personalised Medicine,2009,2(1):77-88.
APA Jing Zhou,Ren-He Xu,&Yong Wang.(2009).Nanoporous membrane-encapsulated feeder cells for culture of human embryonic stem cells.International Journal of Functional Informatics and Personalised Medicine,2(1),77-88.
MLA Jing Zhou,et al."Nanoporous membrane-encapsulated feeder cells for culture of human embryonic stem cells".International Journal of Functional Informatics and Personalised Medicine 2.1(2009):77-88.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jing Zhou]'s Articles
[Ren-He Xu]'s Articles
[Yong Wang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jing Zhou]'s Articles
[Ren-He Xu]'s Articles
[Yong Wang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jing Zhou]'s Articles
[Ren-He Xu]'s Articles
[Yong Wang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.