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Lack of ABCG2 expression and side population properties in human pluripotent stem cells
Hui Zeng1,2; Jung Woo Park1; Min Guo3,4; Ge Lin1; Leann Crandall1; Tiwanna Compton1; Xiaofang Wang1; Xue‐Jun Li3,5; Fang‐Ping Chen2; Ren‐He Xu1,5
2009-10-01
Source PublicationStem Cells
ISSN10665099
Volume27Issue:10Pages:2435-2445
Abstract

The multidrug transporter ABCG2 in cell membranes enables various stem cells and cancer cells to efflux chemicals, including the fluorescent dye Hoechst 33342. The Hoechst cells can be sorted out as a side population with stem cell properties. Abcg2 expression in mouse embryonic stem cells (ESCs) reduces accumulation of DNA-damaging metabolites in the cells, which helps prevent cell differentiation. Surprisingly, we found that human ESCs do not express ABCG2 and cannot efflux Hoechst. In contrast, trophoblasts and neural epithelial cells derived from human ESCs are ABCG2 and Hoechst. Human ESCs ectopically expressing ABCG2 become Hoechst , more tolerant of toxicity of mitoxantrone, a substrate of ABCG2, and more capable of self-renewal in basic fibroblast growth factor (bFGF)-free condition than control cells. However, Hoechst cells sorted as a small subpopulation from human ESCs express lower levels of pluripotency markers than the Hoechst cells. Similar results were observed with human induced pluripotent stem cells. Conversely, mouse ESCs are Abcg2 and mouse trophoblasts, Abcg2. Thus, absence of ABCG2 is a novel feature of human pluripotent stem cells, which distinguishes them from many other stem cells including mouse ESCs, and may be a reason why they are sensitive to suboptimal culture conditions. © AlphaMed Press.

KeywordPluripotent Stem Cells Side Population Trophoblast
DOI10.1002/stem.192
URLView the original
Language英语
WOS Research AreaCell Biology ; Biotechnology & Applied Microbiology ; Oncology ; Hematology
WOS SubjectCell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology
WOS IDWOS:000271830200007
PublisherWILEY, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
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Cited Times [WOS]:34   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorFang‐Ping Chen; Ren‐He Xu
Affiliation1.Department of Genetics and Developmental Biology, University of Connecticut Stem Cell Institute
2.Departments of Hematology, Xiang-Ya Hospital, Central South University, Changsha, Hunan, China
3.Department of Neuroscience, University of Connecticut Health Center, Farmington, Connecticut, USA
4.Departments of Geriatrics, Xiang-Ya Hospital, Central South University, Changsha, Hunan, China
5.University of Connecticut Stem Cell Institute, Farmington, Connecticut, USA
Recommended Citation
GB/T 7714
Hui Zeng,Jung Woo Park,Min Guo,et al. Lack of ABCG2 expression and side population properties in human pluripotent stem cells[J]. Stem Cells,2009,27(10):2435-2445.
APA Hui Zeng.,Jung Woo Park.,Min Guo.,Ge Lin.,Leann Crandall.,...&Ren‐He Xu.(2009).Lack of ABCG2 expression and side population properties in human pluripotent stem cells.Stem Cells,27(10),2435-2445.
MLA Hui Zeng,et al."Lack of ABCG2 expression and side population properties in human pluripotent stem cells".Stem Cells 27.10(2009):2435-2445.
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