UM
Efficient differentiation of human iPSC-derived mesenchymal stem cells to chondroprogenitor cells
Rosa M. Guzzo1; Jason Gibson1; Ren‐He Xu2; Francis Y. Lee3; Hicham Drissi1,2
2013-02-01
Source PublicationJournal of Cellular Biochemistry
ISSN07302312 10974644
Volume114Issue:2Pages:480-490
Abstract

Induced pluripotent stem cells (iPSC) hold tremendous potential for personalized cell-based repair strategies to treat musculoskeletal disorders. To establish human iPSCs as a potential source of viable chondroprogenitors for articular cartilage repair, we assessed the in vitro chondrogenic potential of the pluripotent population versus an iPSC-derived mesenchymal-like progenitor population. We found the direct plating of undifferentiated iPSCs into high-density micromass cultures in the presence of BMP-2 promoted chondrogenic differentiation, however these conditions resulted in a mixed population of cells resembling the phenotype of articular cartilage, transient cartilage, and fibrocartilage. The progenitor cells derived from human iPSCs exhibited immunophenotypic features of mesenchymal stem cells (MSCs) and developed along multiple mesenchymal lineages, including osteoblasts, adipocytes, and chondrocytes in vitro. The data indicate the derivation of a mesenchymal stem cell population from human iPSCs is necessary to limit culture heterogeneity as well as chondrocyte maturation in the differentiated progeny. Moreover, as compared to pellet culture differentiation, BMP-2 treatment of iPSC-derived MSC-like (iPSC-MSC) micromass cultures resulted in a phenotype more typical of articular chondrocytes, characterized by the enrichment of cartilage-specific type II collagen (Col2a1), decreased expression of type I collagen (Col1a1) as well as lack of chondrocyte hypertrophy. These studies represent a first step toward identifying the most suitable iPSC progeny for developing cell-based approaches to repairjoint cartilage damage. © 2012 Wiley Periodicals, Inc.

KeywordChondrogenic Differentiation High-density Culture Human Induced Pluripotent Stem Cells Mesenchymal Stem Cells
DOI10.1002/jcb.24388
URLView the original
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Cell Biology
WOS IDWOS:000312651400025
PublisherWILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
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Cited Times [WOS]:70   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorRosa M. Guzzo; Hicham Drissi
Affiliation1.Department of Orthopaedic Surgery, University of Connecticut Health Center, Farmington, Connecticut
2.Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington,Connecticut
3.Department of Orthopaedic Surgery, Columbia University Medical Center, New York City, New York
Recommended Citation
GB/T 7714
Rosa M. Guzzo,Jason Gibson,Ren‐He Xu,et al. Efficient differentiation of human iPSC-derived mesenchymal stem cells to chondroprogenitor cells[J]. Journal of Cellular Biochemistry,2013,114(2):480-490.
APA Rosa M. Guzzo,Jason Gibson,Ren‐He Xu,Francis Y. Lee,&Hicham Drissi.(2013).Efficient differentiation of human iPSC-derived mesenchymal stem cells to chondroprogenitor cells.Journal of Cellular Biochemistry,114(2),480-490.
MLA Rosa M. Guzzo,et al."Efficient differentiation of human iPSC-derived mesenchymal stem cells to chondroprogenitor cells".Journal of Cellular Biochemistry 114.2(2013):480-490.
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