UM
Establishment of Human cell Type-Specific iPS cells with Enhanced Chondrogenic Potential
Rosa M. Guzzo1,3; Vanessa Scanlon1; Archana Sanjay1; Ren-He Xu2,3,4; Hicham Drissi1,2,3
2014-12
Source PublicationStem Cell Reviews and Reports
ISSN15586804 15508943
Volume10Issue:6Pages:820-829
Abstract

The propensity of induced pluripotent stem (iPS) cells to differentiate into specific lineages may be influenced by a number of factors, including the selection of the somatic cell type used for reprogramming. Herein we report the generation of new iPS cells, which we derived from human articular chondrocytes and from cord blood mononucleocytes via lentiviral-mediated delivery of Oct4, Klf4, Sox2, and cMyc. Molecular, cytochemical, and cytogenic analyses confirmed the acquisition of hallmark features of pluripotency, as well as the retention of normal karyotypes following reprogramming of both the human articular chondrocytes (AC) and the cord blood (CB) cells. In vitro and in vivo functional analyses formally established the pluripotent differentiation capacity of all cell lines. Chondrogenic differentiation assays comparing iPS cells derived from AC, CB, and a well established dermal fibroblast cell line (HDFa-Yk26) identified enhanced proteoglycan-rich matrix formation and cartilage-associated gene expression from AC-derived iPS cells. These findings suggest that the tissue of origin may impact the fate potential of iPS cells for differentiating into specialized cell types, such as chondrocytes. Thus, we generated new cellular tools for the identification of inherent features driving high chondrogenic potential of reprogrammed cells.

KeywordArticular Cartilage Chondrogenic Differentiation Cord Blood Human Induced Pluripotent Stem Cells Reprogramming
DOI10.1007/s12015-014-9538-8
URLView the original
Language英语
WOS Research AreaCell Biology ; Research & Experimental Medicine
WOS SubjectCell & Tissue Engineering ; Cell Biology ; Medicine, Research & Experimental
WOS IDWOS:000344868600007
PublisherHUMANA PRESS INC, 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA
全文获取链接
引用统计
被引频次[WOS]:17   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题University of Macau
Corresponding AuthorRosa M. Guzzo
Affiliation1.Department of Orthopaedic SurgeryUniversity of Connecticut Health CenterFarmingtonUSA
2.Department of Genetics and Developmental BiologyUniversity of Connecticut Health CenterFarmingtonUSA
3.Stem Cell InstituteUniversity of Connecticut Health CenterFarmingtonUSA
4.Faculty of Health SciencesUniversity of MacauTaipaChina
推荐引用方式
GB/T 7714
Rosa M. Guzzo,Vanessa Scanlon,Archana Sanjay,et al. Establishment of Human cell Type-Specific iPS cells with Enhanced Chondrogenic Potential[J]. Stem Cell Reviews and Reports,2014,10(6):820-829.
APA Rosa M. Guzzo,Vanessa Scanlon,Archana Sanjay,Ren-He Xu,&Hicham Drissi.(2014).Establishment of Human cell Type-Specific iPS cells with Enhanced Chondrogenic Potential.Stem Cell Reviews and Reports,10(6),820-829.
MLA Rosa M. Guzzo,et al."Establishment of Human cell Type-Specific iPS cells with Enhanced Chondrogenic Potential".Stem Cell Reviews and Reports 10.6(2014):820-829.
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
Google Scholar
中相似的文章 Google Scholar
[Rosa M. Guzzo]的文章
[Vanessa Scanlon]的文章
[Archana Sanjay]的文章
Baidu academic
中相似的文章 Baidu academic
[Rosa M. Guzzo]的文章
[Vanessa Scanlon]的文章
[Archana Sanjay]的文章
Bing Scholar
中相似的文章 Bing Scholar
[Rosa M. Guzzo]的文章
[Vanessa Scanlon]的文章
[Archana Sanjay]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。