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Mesenchymal stem cells attenuate radiation-induced brain injury by inhibiting microglia pyroptosis
Liao H.1,2; Wang H.1,2; Rong X.1,2; Li E.3; Xu R.-H.3; Peng Y.1,2
2017
Source PublicationBioMed Research International
ISSN23146141
Volume2017
Abstract

Radiation-induced brain injury (RI) commonly occurs in patients who received head and neck radiotherapy. However, the mechanism of RI remains unclear. We aimed to evaluate whether pyroptosis was involved in RI and the impact of mesenchymal stem cells (MSCs) on it. BALB/c male mice (6-8 weeks) were cranially irradiated (15Gy), and MSCs were transplanted into the bilateral cortex 2 days later; thenmice were sacrificed 1 month later. Meanwhile, irradiated BV-2microglia cells (10Gy) were cocultured with MSCs for 24 hours. We observed that irradiated mice brains presented NLRP3 and caspase-1 activation. RT-PCR then indicated that it mainly occurred in microglia cells but not in neurons. Further, irradiated BV-2 cells showed pyroptosis and increased production of IL-18 and IL-1β. RT-PCR also demonstrated an increased expression of several inflammasome genes in irradiated BV- 2 cells, including NLRP3 and AIM2. Particularly, NLRP3 was activated. Knockdown of NLRP3 resulted in decreased LDH release. Noteworthily, in vivo, MSCs transplantation alleviated radiation-inducedNLRP3 and caspase-1 activation. Moreover, in vitro, MSCs could decrease caspase-1 dependent pyroptosis, NLRP3 inflammasome activation, and ROS production induced by radiation. Thus, our findings proved that microglia pyroptosis occurred in RI. MSCs may act as a potent therapeutic tool in attenuating pyroptosis.

DOI10.1155/2017/1948985
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Research & Experimental Medicine
WOS SubjectBiotechnology & Applied Microbiology ; Medicine, Research & Experimental
WOS IDWOS:000417235200001
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Cited Times [WOS]:5   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorPeng Y.
Affiliation1.Department of Neurology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China
2.Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China
3.Faculty of Health Sciences, University of Macau, Taipa, Macau
Recommended Citation
GB/T 7714
Liao H.,Wang H.,Rong X.,et al. Mesenchymal stem cells attenuate radiation-induced brain injury by inhibiting microglia pyroptosis[J]. BioMed Research International,2017,2017.
APA Liao H.,Wang H.,Rong X.,Li E.,Xu R.-H.,&Peng Y..(2017).Mesenchymal stem cells attenuate radiation-induced brain injury by inhibiting microglia pyroptosis.BioMed Research International,2017.
MLA Liao H.,et al."Mesenchymal stem cells attenuate radiation-induced brain injury by inhibiting microglia pyroptosis".BioMed Research International 2017(2017).
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