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Unmasking venom gland transcriptomes in reptile venoms
Chen T.3; Bjourson A.J.3; Orr D.F.3; Kwok H.3; Rao P.2; Ivanyi C.1; Shaw C.3
Source PublicationAnalytical Biochemistry

While structural studies of reptile venom toxins can be achieved using lyophilized venom samples, until now the cloning of precursor cDNAs required sacrifice of the specimen for dissection of the venom glands. Here we describe a simple and rapid technique that unmasks venom protein mRNAs present in lyophilized venom samples. To illustrate the technique we have RT-PCR-amplified a range of venom protein transcripts from cDNA libraries derived from the venoms of a hemotoxic snake, the Chinese copperhead (Deinagkistrodon acutus), a neurotoxic snake, the black mamba (Dendroaspis polylepis), and a venomous lizard, the Gila monster (Heloderma suspectum). These include a metalloproteinase and phospholipase A2 from D. acutus, a potassium channel blocker, dendrotoxin K, from D. polylepis, andexendin-4 from H. suspectum. These findings imply that the apparent absence and/or lability of mRNA in complex biological matrices is not always real and paves the way for accelerated acquisition of molecular genetic data on venom toxins for scientific and potential therapeutic purposes without sacrifice of endangered herpetofauna. © 2002 Elsevier Science (USA). All rights reserved.

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Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical
WOS IDWOS:000179824800006
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Cited Times [WOS]:28   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Arizona Sonora Desert Museum
2.Fuzhou University
3.Ulster University
Recommended Citation
GB/T 7714
Chen T.,Bjourson A.J.,Orr D.F.,et al. Unmasking venom gland transcriptomes in reptile venoms[J]. Analytical Biochemistry,2002,311(2):152-156.
APA Chen T..,Bjourson A.J..,Orr D.F..,Kwok H..,Rao P..,...&Shaw C..(2002).Unmasking venom gland transcriptomes in reptile venoms.Analytical Biochemistry,311(2),152-156.
MLA Chen T.,et al."Unmasking venom gland transcriptomes in reptile venoms".Analytical Biochemistry 311.2(2002):152-156.
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