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Two novel dermaseptin-like antimicrobial peptides with anticancer activities from the skin secretion of Pachymedusa dacnicolor
Shi D.4; Hou X.3; Wang L.4; Gao Y.4; Wu D.4; Xi X.4; Zhou M.4; Kwok H.F.1; Duan J.2; Chen T.4; Shaw C.4
2016-05-12
Source PublicationToxins
ISSN20726651
Volume8Issue:5
Abstract

The dermaseptin antimicrobial peptide family contains members of 27-34 amino acids in length that have been predominantly isolated from the skins/skin secretions of phyllomedusine leaf frogs. By use of a degenerate primer in Rapid amplification of cDNA ends (RACE) PCR designed to a common conserved domain within the 51-untranslated regions of previously-characterized dermaseptin encoding cDNAs, two novel members of this peptide family, named dermaseptin-PD-1 and dermaseptin-PD-2, were identified in the skin secretion of the phyllomedusine frog, Pachymedusa dacnicolor. The primary structures of both peptides were predicted from cloned cDNAs, as well as being confirmed by mass spectral analysis of crude skin secretion fractions resulted from reversed-phase high-performance liquid chromatography. Chemically-synthesized replicates of dermaseptin-PD-1 and dermaseptin-PD-2 were investigated for antimicrobial activity using standard model microorganisms (Gram-positive bacteria, Gram-negative bacteria and a yeast) and for cytotoxicity using mammalian red blood cells. The possibility of synergistic effects between the two peptides and their anti-cancer cell proliferation activities were assessed. The peptides exhibited moderate to high inhibition against the growth of the tested microorganisms and cancer cell lines with low haemolytic activity. Synergistic interaction between the two peptides in inhibiting the proliferation of Escherichia coli and human neuronal glioblastoma cell line, U251MG was also manifested.

KeywordAmphibian Anticancer Antimicrobial Molecular Cloning Peptide
DOI10.3390/toxins8050144
URLView the original
Indexed BySCI
Language英语
WOS Research AreaFood Science & Technology ; Toxicology
WOS SubjectFood Science & Technology ; Toxicology
WOS IDWOS:000377428300025
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Cited Times [WOS]:9   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Universidade de Macau
2.Nanjing University of Traditional Chinese Medicine
3.Second Military Medical University
4.Queen's University Belfast
Recommended Citation
GB/T 7714
Shi D.,Hou X.,Wang L.,et al. Two novel dermaseptin-like antimicrobial peptides with anticancer activities from the skin secretion of Pachymedusa dacnicolor[J]. Toxins,2016,8(5).
APA Shi D..,Hou X..,Wang L..,Gao Y..,Wu D..,...&Shaw C..(2016).Two novel dermaseptin-like antimicrobial peptides with anticancer activities from the skin secretion of Pachymedusa dacnicolor.Toxins,8(5).
MLA Shi D.,et al."Two novel dermaseptin-like antimicrobial peptides with anticancer activities from the skin secretion of Pachymedusa dacnicolor".Toxins 8.5(2016).
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