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In vivo metabolic imaging of insulin with multiphoton fluorescence of human insulin-Au nanodots
Liu C.-L.; Liu T.-M.; Hsieh T.-Y.; Liu H.-W.; Chen Y.-S.; Tsai C.-K.; Chen H.-C.; Lin J.-W.; Hsu R.-B.; Wang T.-D.; Chen C.-C.; Sun C.-K.; Chou P.-T.
2013-06-24
Source PublicationSmall (Weinheim an der Bergstrasse, Germany)
ISSN16136829
Volume9Issue:12Pages:2102-2110
Abstract

Functional human insulin-Au nanodots (NDs) are synthesized for the in vivo imaging of insulin metabolism. Benefiting from its efficient red to near infrared fluorescence, deep tissue subcellular uptake of insulin-Au NDs can be clearly resolved through a least-invasive harmonic generation and two-photon fluorescence (TPF) microscope. In vivo investigations on mice ear and ex vivo assays on human fat tissues conclude that cells with rich insulin receptors have higher uptake of administrated insulin. Interestingly, the insulin-Au NDs can even permeate into lipid droplets (LDs) of adipocytes. Using this newly discovered metabolic phenomenon of insulin, it is found that enlarged adipocytes in type II diabetes mice have higher adjacent/LD concentration contrast with small-sized ones in wild type mice. For human clinical samples, the epicardial adipocytes of patients with diabetes and coronary artery disease (CAD) also show elevated adjacent/LD concentration contrast. As a result, human insulin-Au nanodots provide a new approach to explore subcellular insulin metabolism in model animals or patients with metabolic or cardiovascular diseases.

DOI10.1002/smll.201201887
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS SubjectChemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
WOS IDWOS:000320401800012
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Cited Times [WOS]:17   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
AffiliationNational Taiwan University
Recommended Citation
GB/T 7714
Liu C.-L.,Liu T.-M.,Hsieh T.-Y.,et al. In vivo metabolic imaging of insulin with multiphoton fluorescence of human insulin-Au nanodots[J]. Small (Weinheim an der Bergstrasse, Germany),2013,9(12):2102-2110.
APA Liu C.-L..,Liu T.-M..,Hsieh T.-Y..,Liu H.-W..,Chen Y.-S..,...&Chou P.-T..(2013).In vivo metabolic imaging of insulin with multiphoton fluorescence of human insulin-Au nanodots.Small (Weinheim an der Bergstrasse, Germany),9(12),2102-2110.
MLA Liu C.-L.,et al."In vivo metabolic imaging of insulin with multiphoton fluorescence of human insulin-Au nanodots".Small (Weinheim an der Bergstrasse, Germany) 9.12(2013):2102-2110.
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