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In vivo Quantification of the Structural Changes of Collagens in a Melanoma Microenvironment with Second and Third Harmonic Generation Microscopy
Wu P.-C.; Hsieh T.-Y.; Tsai Z.-U.; Liu T.-M.
2015
Source PublicationScientific Reports
ISSN20452322
Volume5
Abstract

Using in vivo second harmonic generation (SHG) and third harmonic generation (THG) microscopies, we tracked the course of collagen remodeling over time in the same melanoma microenvironment within an individual mouse. The corresponding structural and morphological changes were quantitatively analyzed without labeling using an orientation index (OI), the gray level co-occurrence matrix (GLCM) method, and the intensity ratio of THG to SHG (R THG/SHG). In the early stage of melanoma development, we found that collagen fibers adjacent to a melanoma have increased OI values and SHG intensities. In the late stages, these collagen networks have more directionality and less homogeneity. The corresponding GLCM traces showed oscillation features and the sum of squared fluctuation Var GLCM increased with the tumor sizes. In addition, the THG intensities of the extracellular matrices increased, indicating an enhanced optical inhomogeneity. Multiplying OI, Var GLCM, and R THG/SHG together, the combinational collagen remodeling (CR) index at 4 weeks post melanoma implantation showed a 400-times higher value than normal ones. These results validate that our quantitative indices of SHG and THG microscopies are sensitive enough to diagnose the collagen remodeling in vivo. We believe these indices have the potential to help the diagnosis of skin cancers in clinical practice.

DOI10.1038/srep08879
URLView the original
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000350549400001
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Cited Times [WOS]:23   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
AffiliationNational Taiwan University
Recommended Citation
GB/T 7714
Wu P.-C.,Hsieh T.-Y.,Tsai Z.-U.,et al. In vivo Quantification of the Structural Changes of Collagens in a Melanoma Microenvironment with Second and Third Harmonic Generation Microscopy[J]. Scientific Reports,2015,5.
APA Wu P.-C.,Hsieh T.-Y.,Tsai Z.-U.,&Liu T.-M..(2015).In vivo Quantification of the Structural Changes of Collagens in a Melanoma Microenvironment with Second and Third Harmonic Generation Microscopy.Scientific Reports,5.
MLA Wu P.-C.,et al."In vivo Quantification of the Structural Changes of Collagens in a Melanoma Microenvironment with Second and Third Harmonic Generation Microscopy".Scientific Reports 5(2015).
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