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Detection of malformations in sea urchin plutei exposed to mercuric chloride using different fluorescent techniques
Buttino I.2; Hwang J.-S.4; Romano G.5; Sun C.-K.3; Liu T.-M.3; Pellegrini D.2; Gaion A.2; Sartori D.2
2016
Source PublicationEcotoxicology and Environmental Safety
ISSN10902414 01476513
Volume123Pages:72-80
Abstract

Embryos of Mediterranean sea urchin Paracentrotus lividus and subtropical Echinometra mathaei were exposed to 5,10, 15 and 20μgL and to 1, 2, 3 and 4μgL mercuric chloride (HgCl), respectively. The effective concentration (EC) inducing malformation in 50% of 4-arm pluteus stage (P4) was 16.14μgL for P. lividus and 2.41μgL for E. mathaei. Two-photon (TP), second (SHG) and third harmonic generation (THG) microscopy techniques, TUNEL staining, propidium iodide (PI) and Hoechst 33342 probes were used to detect light signals or to stain apoptotic and necrotic cells in fixed and alive plutei. Signals were detected differently in the two species: TP fluorescence, commonly associated with apoptotic cells, did not increase with increasing HgCl concentrations in P. lividus and in fact, the TUNEL did not reveal induction of apoptosis. PI fluorescence increased in P. lividus in a dose-dependent manner, suggesting a loss of cell permeability. In E. mathaei plutei TP fluorescence increased at increasing HgCl concentrations. THG microscopy revealed skeletal rods in both species. Different fluorescent techniques, used in this study, are proposed as early-warning systems to visualize malformations and physiological responses in sea urchin plutei.

KeywordApoptosis Confocal Microscopy Harmonic Generation Microscopy Skeletal Rod Two-photon Microscopy
DOI10.1016/j.ecoenv.2015.07.027
URLView the original
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEnvironmental Sciences ; Toxicology
WOS IDWOS:000365557500011
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Academic Centre for Innovation and Development in the Food Industry
2.Italian Institute for Environmental Protection and Research
3.National Taiwan University
4.National Taiwan Ocean University
5.Stazione Zoologica Anton Dohrn
Recommended Citation
GB/T 7714
Buttino I.,Hwang J.-S.,Romano G.,et al. Detection of malformations in sea urchin plutei exposed to mercuric chloride using different fluorescent techniques[J]. Ecotoxicology and Environmental Safety,2016,123:72-80.
APA Buttino I..,Hwang J.-S..,Romano G..,Sun C.-K..,Liu T.-M..,...&Sartori D..(2016).Detection of malformations in sea urchin plutei exposed to mercuric chloride using different fluorescent techniques.Ecotoxicology and Environmental Safety,123,72-80.
MLA Buttino I.,et al."Detection of malformations in sea urchin plutei exposed to mercuric chloride using different fluorescent techniques".Ecotoxicology and Environmental Safety 123(2016):72-80.
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