UM
A magnetic bead-based serum proteomic fingerprinting method for parallel analytical analysis and micropreparative purification
Wong M.Y.M.; Yu K.O.Y.; Poon T.C.W.; Ang I.L.; Law M.-K.; Chan K.Y.W.; Ng E.W.Y.; Ngai S.-M.; Sung J.J.Y.; Chan H.L.Y.
2010-05-01
Source PublicationElectrophoresis
ISSN01730835 15222683
Volume31Issue:10Pages:1721-1730
AbstractProteinChip surface-enhanced laser desorption/ionization technology and magnetic beads-based ClinProt system are commonly used for semi-quantitative profiling of plasma proteome in biomarker discovery. Unfortunately, the proteins/peptides detected by MS are non-recoverable. To obtain the protein identity of a MS peak, additional time-consuming and material-consuming purification steps have to be done. In this study, we developed a magnetic beads-based proteomic fingerprinting method that allowed semi-quantitative proteomic profiling and micropreparative purification of the profiled proteins in parallel. The use of different chromatographic magnetic beads allowed us to obtain different proteomic profiles, which were comparable to those obtained by the ProteinChip surface-enhanced laser desorption/ionization technology. Our assays were semi-quantitative. The normalized peak intensity was proportional to concentration measured by immunoassay. Both intra-assay and inter-assay coefficients of variation of the normalized peak intensities were in the range of 4-30%. Our method only required 2 μL of serum or plasma for generating enough proteins for semi-quantitative profiling by MALDI-TOF-MS as well as for gel electrophoresis and subsequent protein identification. The protein peaks and corresponding gel spots could be easily matched by comparing their intensities and masses. Because of its high efficiency and reproducibility, our method has great potentials in clinical research, especially in biomarker discovery. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
KeywordBiomarker Chromatographic magnetic bead MALDI-TOF-MS Plasma proteome Semi-quantitative proteomic profiling
DOI10.1002/elps.200900571
URLView the original
Language英語
Fulltext Access
Citation statistics
Cited Times [WOS]:13   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
AffiliationPrince of Wales Hospital Hong Kong
Recommended Citation
GB/T 7714
Wong M.Y.M.,Yu K.O.Y.,Poon T.C.W.,et al. A magnetic bead-based serum proteomic fingerprinting method for parallel analytical analysis and micropreparative purification[J]. Electrophoresis,2010,31(10):1721-1730.
APA Wong M.Y.M..,Yu K.O.Y..,Poon T.C.W..,Ang I.L..,Law M.-K..,...&Chan H.L.Y..(2010).A magnetic bead-based serum proteomic fingerprinting method for parallel analytical analysis and micropreparative purification.Electrophoresis,31(10),1721-1730.
MLA Wong M.Y.M.,et al."A magnetic bead-based serum proteomic fingerprinting method for parallel analytical analysis and micropreparative purification".Electrophoresis 31.10(2010):1721-1730.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wong M.Y.M.]'s Articles
[Yu K.O.Y.]'s Articles
[Poon T.C.W.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wong M.Y.M.]'s Articles
[Yu K.O.Y.]'s Articles
[Poon T.C.W.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wong M.Y.M.]'s Articles
[Yu K.O.Y.]'s Articles
[Poon T.C.W.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.