UM
Association of hemopexin in tear film and conjunctival macrophages with vernal keratoconjunctivitis
Pong J.C.F.1; Chu C.Y.2; Li W.Y.1; Tang L.Y.2; Li L.2; Lui W.T.2; Poon T.C.W.2; Rao S.K.1; Lam D.S.C.1; Wang C.C.2; Pang C.P.1
2011-04-01
Source PublicationArchives of Ophthalmology
ISSN00039950 15383601
Volume129Issue:4Pages:453-461
AbstractObjective: Vernal keratoconjunctivitis (VKC) is a chronic allergic inflammatory disease with unclear etiology and pathogenesis. We investigated the tear film proteome of patients with VKC to understand the pathologic characteristics of VKC. Methods: Tear samples were collected from healthy volunteers and patients with VKC. Electrophoresis was performed to display the tear proteomic profiles according to VKC severity. The identities of differentially expressed proteins were analyzed by mass spectrometry and quantified by enzyme-linked immunosorbent assay. Impression cytology was performed on VKC conjunctival samples to demonstrate the cellular protein expression. Allergic sensitization was performed in mice to study the pathologic role of these proteins in VKC. Results: Hemopexin, an inflammatory protein, was elevated in the tear film of patients with VKC. The increased hemopexin concentration in VKC tears was significantly associated with disease severity. Impression cytology showed specific high hemopexin expression in dekeratinized conjunctival epithelium and necrotic macrophages in patients with VKC. Immunohistochemical examination of normal lacrimal tissues from mice showed that hemopexin was not expressed in any lacrimal apparatus. Under systemic and topical sensitization and challenge using hemopexin in mice, the affected eye had mild to moderate bead discharge, chemosis, and edema with excessive macrophage infiltration and conjunctival necrosis. Conclusion: An association exists between tear hemopexin and the development and pathologic effects of VKC. Clinical Relevance: Increased hemopexin may have a role in the development of VKC. © 2011 American Medical Association. All rights reserved.
DOI10.1001/archophthalmol.2011.41
URLView the original
Language英語
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被引频次[WOS]:10   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题University of Macau
Affiliation1.Chinese University of Hong Kong
2.Prince of Wales Hospital Hong Kong
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Pong J.C.F.,Chu C.Y.,Li W.Y.,et al. Association of hemopexin in tear film and conjunctival macrophages with vernal keratoconjunctivitis[J]. Archives of Ophthalmology,2011,129(4):453-461.
APA Pong J.C.F..,Chu C.Y..,Li W.Y..,Tang L.Y..,Li L..,...&Pang C.P..(2011).Association of hemopexin in tear film and conjunctival macrophages with vernal keratoconjunctivitis.Archives of Ophthalmology,129(4),453-461.
MLA Pong J.C.F.,et al."Association of hemopexin in tear film and conjunctival macrophages with vernal keratoconjunctivitis".Archives of Ophthalmology 129.4(2011):453-461.
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