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Multi-wavelength spectrophotometric determination of acid dissociation constants: A validation study
Tam K.Y.1; Takacs-Novak K.2
2001-04-25
Source PublicationAnalytica Chimica Acta
ISSN00032670
Volume434Issue:1Pages:157-167
Abstract

The pK values of 25 structurally diverse drug compounds including mono-, di- and tri-protic molecules were determined by a recently developed multi-wavelength spectrophotometric technique, called dip-probe absorption spectroscopy (D-PAS), and the traditional spectrophotometric titration. All experiments were performed at 25.0 ± 0.5°C in an ionic strength of 0.15 M KCl in two laboratories (Semmelweis and Sirius), to evaluate the reproducibility and reliability of the spectrophotometric techniques for pK determination. It was found that the pK values determined by the D-PAS technique are in excellent agreement with values measured by the traditional spectrophotometric method (average deviation = 0.08 units) and are consistent with the literature data where available. We have demonstrated that the D-PAS approach is a simple, precise and easy-to-use method for pK determination of drug molecules with ionisable group(s) in proximity to chromophore(s). The advantages and limitations of spectrophotometric pK determination are discussed © 2001 Elsevier Science B.V.

KeywordAcid Dissociation Dip-probe Absorption Spectroscopy (D-pas) Spectrophotometric Titration
DOI10.1016/S0003-2670(01)00810-8
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000168287200019
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被引频次[WOS]:102   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题Faculty of Health Sciences
Affiliation1.Sirius Analytical Instruments Ltd.
2.Semmelweis Egyetem
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Tam K.Y.,Takacs-Novak K.. Multi-wavelength spectrophotometric determination of acid dissociation constants: A validation study[J]. Analytica Chimica Acta,2001,434(1):157-167.
APA Tam K.Y.,&Takacs-Novak K..(2001).Multi-wavelength spectrophotometric determination of acid dissociation constants: A validation study.Analytica Chimica Acta,434(1),157-167.
MLA Tam K.Y.,et al."Multi-wavelength spectrophotometric determination of acid dissociation constants: A validation study".Analytica Chimica Acta 434.1(2001):157-167.
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