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Permeation of a fully ionized species across a polarized supported liquid membrane
Velicky M.1; Tam K.Y.2; Dryfe R.A.W.1
2012-03-06
Source PublicationAnalytical Chemistry
ISSN00032700
Volume84Issue:5Pages:2541-2547
Abstract

An analytical technique for the detection of permeation of a fully ionized analyte across a lipophilic membrane is reported. The system, which is comprised of two aqueous compartments (donor and acceptor) separated by a supported liquid membrane, is based on the parallel artificial membrane permeation assay (PAMPA), widely used in the drug discovery process to estimate permeability in vivo. The in situ spectroelectrochemical method developed here employs mechanical stirring of the solution phases on either side of the membrane, external polarization of the membrane, and in situ detection of the analyte via UV-vis spectrophotometry. The flux of the crystal violet cation across the membrane is simultaneously measured via UV-vis spectrophotometry and voltammetry/chronoamperometry as a function of applied potential. The relative contribution of two permeation modes, i.e., that due to naked ions and ion-pairs, is thereby quantified. The open circuit potential difference between the two aqueous compartments and the cyclic voltammetric response are also recorded as a function of time and compared with the predicted values. © 2012 American Chemical Society.

DOI10.1021/ac300016n
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000301021400062
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Cited Times [WOS]:22   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.University of Manchester
2.AstraZeneca
Recommended Citation
GB/T 7714
Velicky M.,Tam K.Y.,Dryfe R.A.W.. Permeation of a fully ionized species across a polarized supported liquid membrane[J]. Analytical Chemistry,2012,84(5):2541-2547.
APA Velicky M.,Tam K.Y.,&Dryfe R.A.W..(2012).Permeation of a fully ionized species across a polarized supported liquid membrane.Analytical Chemistry,84(5),2541-2547.
MLA Velicky M.,et al."Permeation of a fully ionized species across a polarized supported liquid membrane".Analytical Chemistry 84.5(2012):2541-2547.
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