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Geniposide improves insulin production and reduces apoptosis in high glucose-induced glucotoxic insulinoma cells
Guo L.X.1; Liu J.H.1,2; Zheng X.X.1; Yin Z.Y.1; Kosaraju J.3; Tam K.Y.3
2017-12-15
Source PublicationEuropean Journal of Pharmaceutical Sciences
ISSN18790720 09280987
Volume110Pages:70-76
Abstract

Our previous work revealed that in the pancreatic β cell line, geniposide modulated ATP production and glucose-stimulated insulin secretion (GSIS) induced by the acute stimulation of high glucose concentration. However, the effects of geniposide on functional impairment and the mass of β-cells exposed to elevated levels of glucose remains unknown. In the present study, impaired GSIS and restrained proliferation were observed in the prolonged culture of insulinoma INS-1 cells with 33 mM of glucose (high glucose). Our results indicate that the glucose-induced impairment of insulin release was significantly reverted by the inclusion of 1 or 10 μM of geniposide. Moreover, induction of the phosphorylation of AMP-activated protein kinase (AMPK) was observed, which promoted the utilization of nutrient stores for energy production. AMPK phosphorylation was enhanced by an increased number of INS-1 cells, and the increased expression of AMPK downstream target heme oxygenase 1 (HO-1), under high glucose concentration. Furthermore, geniposide protected rat insulinoma cells from apoptosis in high-glucose concentrations. We have shown that these effects were associated with an increased apoptosis-related Bcl-2/BAX protein ratio. In conclusion, geniposide dose dependently improves β-cell function and increases the proliferation of β-cells exposed to prolonged hyperglycemia.

KeywordApoptosis Geniposide Glucose-stimulated Insulin Secretion Glucotoxicity
DOI10.1016/j.ejps.2017.03.038
URLView the original
Indexed BySCI
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000414696100009
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Cited Times [WOS]:5   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Chongqing Key Lab of Natural Medicine Research, Chongqing Technology and Business University, Chongqing 400067, China
2.College of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400050, China
3.Faculty of Health Sciences, University of Macau, Taipa, Macau, China
Recommended Citation
GB/T 7714
Guo L.X.,Liu J.H.,Zheng X.X.,et al. Geniposide improves insulin production and reduces apoptosis in high glucose-induced glucotoxic insulinoma cells[J]. European Journal of Pharmaceutical Sciences,2017,110:70-76.
APA Guo L.X.,Liu J.H.,Zheng X.X.,Yin Z.Y.,Kosaraju J.,&Tam K.Y..(2017).Geniposide improves insulin production and reduces apoptosis in high glucose-induced glucotoxic insulinoma cells.European Journal of Pharmaceutical Sciences,110,70-76.
MLA Guo L.X.,et al."Geniposide improves insulin production and reduces apoptosis in high glucose-induced glucotoxic insulinoma cells".European Journal of Pharmaceutical Sciences 110(2017):70-76.
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