UM
Differential screening of a rat entorhinal cortex cDNA library for clozapine regulated genes
Linden A.-M.; Vaisanen J.; Bichet S.; Wong G.; Castren E.
1997-12-01
Source PublicationFASEB Journal
ISSN08926638
Volume11Issue:9
AbstractClozapine is a prototype of atypical antipsychotics and the most effective treatment for schizophrenia. Genes that are activated or repressed during long term administration of clozapine will provide new information about mechanisms of its antipsychotic actions. In order to investigate the effects of chronic administration of clozapine (i.p. ; 25 mg/kg; 17 days) on genes expressed in the entorhinal cortex, we have differentially screened a eDNA library (pBluescript), which was constructed from mRNAs isolated from clozapine treated rat entorhinal cortices. More than 1000 eDNA clones were randomly picked from the library, duplicate southern blotted onto filters and reverse northern hybridized with 32p labelled control and clozapine eDNA probes synthesized from mRNAs of saline (control) and clozapine treated rat entorhinal cortices. The differential screening procedure revealed 13 candidate cDNAs regulated by clozapine. To confirm the up- or downregulation and regional specific expression of cDNAs we are in situ hybridizing brain sections of saline and clozapine treated rats with cRNA probes generated from the candidate cDNAs. The sequence and identification of the most interesting genes could help to reveal the mechanisms of the actions of ctozapine.
URLView the original
Language英語
Fulltext Access
Document TypeJournal article
CollectionUniversity of Macau
AffiliationItä-Suomen yliopisto
Recommended Citation
GB/T 7714
Linden A.-M.,Vaisanen J.,Bichet S.,et al. Differential screening of a rat entorhinal cortex cDNA library for clozapine regulated genes[J]. FASEB Journal,1997,11(9).
APA Linden A.-M.,Vaisanen J.,Bichet S.,Wong G.,&Castren E..(1997).Differential screening of a rat entorhinal cortex cDNA library for clozapine regulated genes.FASEB Journal,11(9).
MLA Linden A.-M.,et al."Differential screening of a rat entorhinal cortex cDNA library for clozapine regulated genes".FASEB Journal 11.9(1997).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Linden A.-M.]'s Articles
[Vaisanen J.]'s Articles
[Bichet S.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Linden A.-M.]'s Articles
[Vaisanen J.]'s Articles
[Bichet S.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Linden A.-M.]'s Articles
[Vaisanen J.]'s Articles
[Bichet S.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.