UM
Expression of the naturally occurring truncated trkB neurotrophin receptor induces outgrowth of filopodia and processes in neuroblastoma cells
Haapasalo A.1; Saarelainen T.1; Moshnyakov M.2; Arumae U.2; Kiema T.-R.1; Saarma M.2; Wong G.1; Castren E.1
1999-02-11
Source PublicationOncogene
ISSN09509232
Volume18Issue:6Pages:1285-1296
AbstractWe have investigated the effects of the truncated trkB receptor isoform T1 (trkB.T1) by transient transfection into mouse N2a neuroblastoma cells. We observed that expression of trkB.T1 leads to a striking change in cell morphology characterized by outgrowth of filopodia and processes. A similar morphological response was also observed in SH-SY5Y human neuroblastoma cells and NIH3T3 fibroblasts transfected with trkB.T1, N2a cells lack endogenous expression of trkB isoforms, but express barely detectable amounts of its ligands, brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT-4). The morphological change was ligand-independent, since addition of exogenous BDNF or NT-4 or blockade of endogenous trkB ligands did not influence this response. Filopodia and process outgrowth was significantly suppressed when full-length trkB.TK + was cotransfected together with trkB.T1 and this inhibitory effect was blocked by tyrosine kinase inhibitor K252a. Transfection of trkB.T1 deletion mutants showed that the morphological response is dependent on the extracellular, but not the intracellular domain of the receptor. Our results suggest a novel ligand-independent role for truncated trkB in the regulation of cellular morphology.
KeywordBDNF N2a Neuroblastoma NT-4 trkB
DOI10.1038/sj.onc.1202401
URLView the original
Language英語
全文获取链接
引用统计
被引频次[WOS]:53   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题University of Macau
Affiliation1.Itä-Suomen yliopisto
2.Helsingin Yliopisto
推荐引用方式
GB/T 7714
Haapasalo A.,Saarelainen T.,Moshnyakov M.,et al. Expression of the naturally occurring truncated trkB neurotrophin receptor induces outgrowth of filopodia and processes in neuroblastoma cells[J]. Oncogene,1999,18(6):1285-1296.
APA Haapasalo A..,Saarelainen T..,Moshnyakov M..,Arumae U..,Kiema T.-R..,...&Castren E..(1999).Expression of the naturally occurring truncated trkB neurotrophin receptor induces outgrowth of filopodia and processes in neuroblastoma cells.Oncogene,18(6),1285-1296.
MLA Haapasalo A.,et al."Expression of the naturally occurring truncated trkB neurotrophin receptor induces outgrowth of filopodia and processes in neuroblastoma cells".Oncogene 18.6(1999):1285-1296.
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
Google Scholar
中相似的文章 Google Scholar
[Haapasalo A.]的文章
[Saarelainen T.]的文章
[Moshnyakov M.]的文章
Baidu academic
中相似的文章 Baidu academic
[Haapasalo A.]的文章
[Saarelainen T.]的文章
[Moshnyakov M.]的文章
Bing Scholar
中相似的文章 Bing Scholar
[Haapasalo A.]的文章
[Saarelainen T.]的文章
[Moshnyakov M.]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。