UM
Induction of cAMP response element modulator (CREM) and inducible cAMP early repressor (ICER) expression in rat brain by uncompetitive N-methyl-D- aspartate receptor antagonists
Storvik M.; Linden A.-M.; Kontkanen O.; Lakso M.; Castren E.; Wong G.
2000-07-01
Source PublicationJournal of Pharmacology and Experimental Therapeutics
ISSN00223565
Volume294Issue:1Pages:52-60
AbstractThe N-methyl-D-aspartate (NMDA) subtype of glutamate receptor mediates fast excitatory neurotransmission, and agents that attenuate this function are neuroprotective, anesthetic, and psychotropic. To determine whether cAMP regulatable transcription factors play a role in the neurochemical actions of agents acting through NMDA receptors, the effects of the acute administration of uncompetitive and competitive antagonists on the expression of cAMP response element modulator (CREM) and inducible cAMP early repressor (ICER) transcription factors were examined. In situ hybridization to rat brain sections revealed that ICER mRNA expression was significantly increased by uncompetitive NMDA receptor antagonists (MK-801, phencyclidine, ketamine, memantine) but not by the competitive antagonist CPP [(±)-3-(2- carboxypiperazin-4-yl)-propyl-1-phosphonic acid] or other psychotropic agents (clozapine, haloperidol, desipramine). Major brain regions where ICER transcripts were increased were the hippocampus, parietal cortex layers IV and VI, temporal cortex, cingulate cortex, thalamus, and granule cell layer of the olfactory bulb. Northern and Western blot analyses indicated that CREM mRNA and protein, respectively, were also increased after MK-801 treatment, but ICER isoforms predominate during both basal and induced conditions. MK- 801 also transiently increased the binding of proteins to cAMP response element, which was supershifted by anti-CREM antibody, indicating that increased mRNA and protein levels have functional consequences on gene transcription. These results provide evidence for the involvement of CREM and ICER family transcription factors in the pharmacologic effects of uncompetitive NMDA receptor antagonists.
URLView the original
Language英語
Fulltext Access
Document TypeJournal article
CollectionUniversity of Macau
AffiliationItä-Suomen yliopisto
Recommended Citation
GB/T 7714
Storvik M.,Linden A.-M.,Kontkanen O.,et al. Induction of cAMP response element modulator (CREM) and inducible cAMP early repressor (ICER) expression in rat brain by uncompetitive N-methyl-D- aspartate receptor antagonists[J]. Journal of Pharmacology and Experimental Therapeutics,2000,294(1):52-60.
APA Storvik M.,Linden A.-M.,Kontkanen O.,Lakso M.,Castren E.,&Wong G..(2000).Induction of cAMP response element modulator (CREM) and inducible cAMP early repressor (ICER) expression in rat brain by uncompetitive N-methyl-D- aspartate receptor antagonists.Journal of Pharmacology and Experimental Therapeutics,294(1),52-60.
MLA Storvik M.,et al."Induction of cAMP response element modulator (CREM) and inducible cAMP early repressor (ICER) expression in rat brain by uncompetitive N-methyl-D- aspartate receptor antagonists".Journal of Pharmacology and Experimental Therapeutics 294.1(2000):52-60.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Storvik M.]'s Articles
[Linden A.-M.]'s Articles
[Kontkanen O.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Storvik M.]'s Articles
[Linden A.-M.]'s Articles
[Kontkanen O.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Storvik M.]'s Articles
[Linden A.-M.]'s Articles
[Kontkanen O.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.