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Qualitation and quantification of water soluble non-starch polysaccharides from Pseudostellaria heterophylla in China using saccharide mapping and multiple chromatographic methods
Deng, Yong1; Han, Bang-Xing2; Hu, De-Jun1; Zhao, Jing1; Li, Shao-Ping1
2018-11
Source PublicationCARBOHYDRATE POLYMERS
ISSN0144-8617
Volume199Pages:619-627
Abstract

Qualitative and quantitative analysis of non-starch polysaccharide (NSP) from Pseudostellaria heterophylla in China (Guizhou, Anhui and Fujian) were performed using high performance size exclusion chromatography coupled with multi-angle laser light scattering and refractive index detector (HPSEC-MALLS-RID), saccharide mapping based on polysaccharide analysis by using carbohydrate gel electrophoresis (PACE) and high performance thin layer chromatography (HPTLC). Results showed that PACE and HPTLC fingerprints of enzymatic hydrolysates of NSP were similar. Moreover, the results indicated that 1,4-beta-d-Galp, 1,5-alpha-Araf, 1,4-alpha-d-GalAp and 1,4-beta-d-Glcp linkages existed in NSP. Based on the results of HPSEC-MALLS/RI, the Mw, Rg, and contents of NSP in P. heterophylla were ranging from 4.37x10(4) to 1.70x10(5) Da, 46.4 to 65.9 nm, and 0.43% to 1.41%, respectively. There were no significant difference (p > 0.05) observed among the three main producing areas, which suggested that polysaccharides could be used as marker for quality control of P. heterophylla.

KeywordPseudostellaria Heterophylla Polysaccharides Saccharide Mapping Hpsec-malls-rid Pace Hptlc
DOI10.1016/j.carbpol.2018.06.063
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Polymer Science
WOS SubjectChemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS IDWOS:000442348500067
PublisherELSEVIER SCI LTD
The Source to ArticleWOS
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Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macau, Peoples R China
2.West Anhui Univ, Coll Biol & Pharmaceut Engn, Luan, Anhui, Peoples R China
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Deng, Yong,Han, Bang-Xing,Hu, De-Jun,et al. Qualitation and quantification of water soluble non-starch polysaccharides from Pseudostellaria heterophylla in China using saccharide mapping and multiple chromatographic methods[J]. CARBOHYDRATE POLYMERS,2018,199:619-627.
APA Deng, Yong,Han, Bang-Xing,Hu, De-Jun,Zhao, Jing,&Li, Shao-Ping.(2018).Qualitation and quantification of water soluble non-starch polysaccharides from Pseudostellaria heterophylla in China using saccharide mapping and multiple chromatographic methods.CARBOHYDRATE POLYMERS,199,619-627.
MLA Deng, Yong,et al."Qualitation and quantification of water soluble non-starch polysaccharides from Pseudostellaria heterophylla in China using saccharide mapping and multiple chromatographic methods".CARBOHYDRATE POLYMERS 199(2018):619-627.
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