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Title: Comparison and characterization of polysaccharides from natural and cultured Cordyceps using saccharide mapping
Authors: Zhao, Jing (趙靜)
Li, Shao Ping (李紹平)
Guan, Jia
Feng, Kun
Hu, De Jun (胡德俊)
Issue Date: 10-Nov-2011
Publisher: Springer
Citation: Analytical and bioanalytical chemistry, 10 Nov. 2011, no. 399, p. 3465-3474
Abstract: Comparison and characterization of polysaccharides from natural and cultured Cordyceps on the basis of their chemical characteristics such as glycosidic linkages were performed for the first time using saccharide mapping. The results showed that polysaccharides from most of the natural and cultured Cordyceps had similar responses to enzymatic digestion. These polysaccharides mainly contained (1→4)-β-D-glucosidic linkages, and (1→4)-α-glucosidic, (1→6)-α-glucosidic, 1,4-β-D-mannosidic, as well as (1→4)-α-D-galactosiduronic linkages also existed in some polysaccharides. Especially, natural and cultured Cordyceps polysaccharides could be discriminated on the basis of high performance liquid chromatography profiles of pectinase hydrolysates, which is helpful to control the quality of polysaccharides from Cordyceps.
ISSN: 1618-2642
Keywords: Cordyceps
Enzymatic digestion
Saccharide mapping
High-performance size-exclusion chromatography
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