UM
Transcriptional profiling reveals differential expression of a neuropeptide-like protein and pseudogenes in aryl hydrocarbon receptor-1 mutant Caenorhabditis elegans
Aarnio V.; Heikkinen L.; Peltonen J.; Goldsteins G.; Lakso M.; Wong G.
2014-03-01
Source PublicationComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
ISSN1744117X 18780407
Volume9Issue:1Pages:40-48
AbstractThe aryl hydrocarbon receptor (AHR) functions in higher organisms in development, metabolism and toxic responses. Its Caenorhabditis elegans (C. elegans) ortholog, AHR-1, facilitates neuronal development, growth and movement. We investigated the effect of AHR mutation on the transcriptional profile of L4 stage C. elegans using RNA-seq and quantitative real time PCR in order to understand better AHR-1 function at the genomic level. Illumina HiSeq 2000 sequencing yielded 51.1, 61.2 and 54.0 million reads from wild-type controls, ahr-1(ia03) and ahr-1(ju145) mutants, respectively, providing detection of over 18,000 transcripts in each sample. Fourteen transcripts were over-expressed and 125 under-expressed in both ahr-1 mutants when compared to wild-type. Under-expressed genes included soluble guanylate cyclase (gcy) family genes, some of which were previously demonstrated to be regulated by AHR-1. A neuropeptide-like protein gene, nlp-20, and a F-box domain protein gene fbxa-192 and its pseudogenes fbxa-191 and fbxa-193 were also under-expressed. Conserved xenobiotic response elements were identified in the 5′ flanking regions of some but not all of the gcy, nlp-20, and fbxa genes. These results extend previous studies demonstrating control of gcy family gene expression by AHR-1, and furthermore suggest a role of AHR-1 in regulation of a neuropeptide gene as well as pseudogenes. © 2014 Elsevier Inc. All rights reserved.
KeywordAryl hydrocarbon receptor C. elegans Neuropeptide Pseudogene RNA-seq
DOI10.1016/j.cbd.2013.12.001
URLView the original
Language英語
Fulltext Access
Citation statistics
Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
AffiliationItä-Suomen yliopisto
Recommended Citation
GB/T 7714
Aarnio V.,Heikkinen L.,Peltonen J.,et al. Transcriptional profiling reveals differential expression of a neuropeptide-like protein and pseudogenes in aryl hydrocarbon receptor-1 mutant Caenorhabditis elegans[J]. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics,2014,9(1):40-48.
APA Aarnio V.,Heikkinen L.,Peltonen J.,Goldsteins G.,Lakso M.,&Wong G..(2014).Transcriptional profiling reveals differential expression of a neuropeptide-like protein and pseudogenes in aryl hydrocarbon receptor-1 mutant Caenorhabditis elegans.Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics,9(1),40-48.
MLA Aarnio V.,et al."Transcriptional profiling reveals differential expression of a neuropeptide-like protein and pseudogenes in aryl hydrocarbon receptor-1 mutant Caenorhabditis elegans".Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics 9.1(2014):40-48.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Aarnio V.]'s Articles
[Heikkinen L.]'s Articles
[Peltonen J.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Aarnio V.]'s Articles
[Heikkinen L.]'s Articles
[Peltonen J.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Aarnio V.]'s Articles
[Heikkinen L.]'s Articles
[Peltonen J.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.