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Ca2+ signal blockers can inhibit M/A transition in mammalian cells by interfering with the spindle checkpoint
Xu N.; Luo K.Q.; Chang D.C.
2003-07-04
Source PublicationBiochemical and Biophysical Research Communications
ISSN0006291X
Volume306Issue:3Pages:737-745
Abstract

A key step in mitosis is the sister-chromatid separation at the metaphase-anaphase (M/A) transition. Several earlier studies had suggested that Ca signal is involved in regulating this process in somatic cells. The detailed mechanisms, however, are not yet well understood. In this study, we used the GFP-gene fusion method and a living-cell imaging technique to examine the effects of suppressing cytosolic Ca level on the mitotic process in HeLa and PtK2 cells. We observed that application of the Ca chelator BAPTA/AM can block or severely delay the M/A transition. This blockage was caused by a failure in activating the anaphase-promoting complex (APC), since both cyclin B and securin could not be degraded under this situation. Furthermore, using YFP-labeled tubulin, we found that the mitotic spindle structure in most of the BAPTA-treated cells gradually deformed with time. Other Ca signal blockers, such as heparin, also produced a similar effect. These results suggest that one pathway for the blockage of M/A transition by suppressing cytosolic Ca level is due to its interference with the mitotic spindle checkpoint. © 2003 Elsevier Science (USA). All rights reserved.

KeywordBapta/am Calcium Cyclin b Metaphase-anaphase Transition Microtubule Mitotic Spindle Securin
DOI10.1016/S0006-291X(03)01061-1
URLView the original
Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000183818900018
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Cited Times [WOS]:27   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
AffiliationHong Kong University of Science and Technology
Recommended Citation
GB/T 7714
Xu N.,Luo K.Q.,Chang D.C.. Ca2+ signal blockers can inhibit M/A transition in mammalian cells by interfering with the spindle checkpoint[J]. Biochemical and Biophysical Research Communications,2003,306(3):737-745.
APA Xu N.,Luo K.Q.,&Chang D.C..(2003).Ca2+ signal blockers can inhibit M/A transition in mammalian cells by interfering with the spindle checkpoint.Biochemical and Biophysical Research Communications,306(3),737-745.
MLA Xu N.,et al."Ca2+ signal blockers can inhibit M/A transition in mammalian cells by interfering with the spindle checkpoint".Biochemical and Biophysical Research Communications 306.3(2003):737-745.
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