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Extraction and purification of isoflavones from soybeans and characterization of their estrogenic activities
Zhang E.J.; Ka M.N.; Luo K.Q.
2007-08-22
Source PublicationJournal of Agricultural and Food Chemistry
ISSN00218561
Volume55Issue:17Pages:6940-6950
Abstract

Soybean isoflavones have multiple beneficial health effects especially on estrogen-deficient diseases such as menopausal symptoms. In this study, isoflavones were produced from soybean flour, and the extraction and purification parameters were optimized to give a high yield of total isoflavones, about 0.62 mg of aglycones/g of soybean flour, which is >2 times the initial yield. HPLC analysis and MTT cell proliferation assay using MCF-7 cells revealed that the product thus obtained not only contained a high content of isoflavone aglycones but also had estrogenic activity. MTT data also revealed that both genistein and daidzein exhibited estrogenic effects at lower concentrations and antiproliferative effects at higher concentrations, and 1 μM genistein and 10 μM daidzein exerted significant estrogenic activities, which were not more than that of the endogenous level of 17β-estradiol (E). The production method developed can be used as a guideline for manufacturing soy isoflavones, and the MTT assay was demonstrated to be suitable for quality control on isoflavone products. The results on the estrogenic properties of isoflavones can be used as reference data for their effective and safe usages in estrogenic therapy. © 2007 American Chemical Society.

KeywordDaidzein Estrogenic Properties Extraction Genistein Isoflavones Mtt Assay Optimization Purification Quality Control Soybeans
DOI10.1021/jf0708903
URLView the original
Indexed BySCI
Language英语
WOS Research AreaAgriculture ; Chemistry ; Food Science & Technology
WOS SubjectAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS IDWOS:000248727800020
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Cited Times [WOS]:59   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
AffiliationHong Kong University of Science and Technology
Recommended Citation
GB/T 7714
Zhang E.J.,Ka M.N.,Luo K.Q.. Extraction and purification of isoflavones from soybeans and characterization of their estrogenic activities[J]. Journal of Agricultural and Food Chemistry,2007,55(17):6940-6950.
APA Zhang E.J.,Ka M.N.,&Luo K.Q..(2007).Extraction and purification of isoflavones from soybeans and characterization of their estrogenic activities.Journal of Agricultural and Food Chemistry,55(17),6940-6950.
MLA Zhang E.J.,et al."Extraction and purification of isoflavones from soybeans and characterization of their estrogenic activities".Journal of Agricultural and Food Chemistry 55.17(2007):6940-6950.
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