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Bioassay guided discovery of apoptosis inducers from gamboge by high-speed counter-current chromatography and high-pressure liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry
Han Q.-B.2; Zhou Y.2; Feng C.1; Xu G.2; Huang S.-X.2; Li S.-L.2; Qiao C.-F.2; Song J.-Z.2; Chang D.C.1; Luo K.Q.1; Xu H.-X.2
2009-02-01
Source PublicationJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ISSN15700232
Volume877Issue:4Pages:401-407
Abstract

A screening system, composed of high-speed counter-current chromatography and high-pressure liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry, was established to find bioactive lead compound. This system succeeded in discovering apoptosis inducers from gamboge, the resin of Garcinia hanburyi. High-speed counter-current chromatography was used to provide well-separated fractions for bioassay and the resulted active fractions were rapidly identified using high-pressure liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry. The solvent system of n-hexane/ethyl acetate/methanol/water was optimized to the ratio of 7:3:7:3 (v/v/v/v) by a K value analysis. As a result, two active fractions were obtained. They showed apoptosis inducing effects as potent as that of taxol (500 nM) at the concentration of 1 μg/ml. Gambogenic acid (72.1%) and epimeric isogambogic acids (25.3%) were identified in one of the fractions. The other active fraction mainly contained two epimeric mixtures, gambogic acids (68.7%) and gambogoic acids (26.9%). Among them, gambogenic acid, without epimerization, has priority to be lead compound. © 2008 Elsevier B.V. All rights reserved.

KeywordApoptosis Inducer Gambogenic Acid Garcinia Hanburyi High-speed Counter-current Chromatography Hplc/ms
DOI10.1016/j.jchromb.2008.12.046
URLView the original
Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000263190900007
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Cited Times [WOS]:30   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Hong Kong University of Science and Technology
2.Hong Kong Jockey Club Institute of Chinese Medicine
Recommended Citation
GB/T 7714
Han Q.-B.,Zhou Y.,Feng C.,et al. Bioassay guided discovery of apoptosis inducers from gamboge by high-speed counter-current chromatography and high-pressure liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry[J]. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences,2009,877(4):401-407.
APA Han Q.-B..,Zhou Y..,Feng C..,Xu G..,Huang S.-X..,...&Xu H.-X..(2009).Bioassay guided discovery of apoptosis inducers from gamboge by high-speed counter-current chromatography and high-pressure liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry.Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences,877(4),401-407.
MLA Han Q.-B.,et al."Bioassay guided discovery of apoptosis inducers from gamboge by high-speed counter-current chromatography and high-pressure liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry".Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 877.4(2009):401-407.
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