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Quick identification of apoptosis inducer from Isodon eriocalyx by a drug discovery platform composed of analytical high-speed counter-current chromatography and the fluorescence-based caspase-3 biosensor detection
Han Q.-B.4; Yu T.3; Lai F.1; Zhou Y.1; Feng C.3; Wang W.-N.2; Fu X.-H.2; Lau C.B.-S.4; Luo K.Q.3; Xu H.-X.1; Sun H.-D.5; Fung K.-P.4; Leung P.-C.4
2010-09-15
Source PublicationTalanta
ISSN00399140
Volume82Issue:4Pages:1521-1527
Abstract

Analytical high-speed counter-current chromatography (HSCCC), a unique liquid-to-liquid separation technology, has an inherent capability to provide perfect fractionation for tracking active ingredients of medicinal herbs, in a quick, efficient, and high-recovery manner. A high throughput screening (HTS) method which utilizes a novel biosensor that selectively detects apoptosis based on the fluorescence resonance energy transfer (FRET) technique, was newly established and proved to be very sensitive in detecting apoptosis induced by various known anticancer drugs. The first combination of both advanced techniques formed an efficient platform for drug discovery and succeeded in quickly identifying the most potent apoptotic constituent of a Chinese herb namely Isodon eriocalyx. The system of n-hexane/ethyl acetate/methanol/water was used as the separation solvent. The solvent ratio was first set at 3:5:3:5 to check the water-soluble part of the crude extract, and then 1:1:1:1 was used to isolate the target compounds. The active fraction was tracked and purified continuously using HSCCC which was guided by the apoptosis detection at gradually decreased drug concentrations. As a result, the most potent apoptosis inducer in this herb was discovered by analytical HSCCC equipped with a 16 ml mini-coil column, using less than 50 ml diphase solvent, from about 50 mg active fraction. It was identified as eriocalyxin B, a well-known antitumor natural product, by NMR analysis of the HSCCC purified fraction. © 2010 Elsevier B.V. All rights reserved.

KeywordAnalytical High-speed Counter-current Chromatography Apoptosis Inducer Caspase-3 Biosensor Eriocalyxin b Isodon Eriocalyx
DOI10.1016/j.talanta.2010.07.036
URLView the original
Indexed BySCI
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000282480500066
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Cited Times [WOS]:23   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Hong Kong Jockey Club Institute of Chinese Medicine
2.Shanghai Tauto Biotech Co., Ltd.
3.Nanyang Technological University
4.Chinese University of Hong Kong
5.Kunming Institute of Botany Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Han Q.-B.,Yu T.,Lai F.,et al. Quick identification of apoptosis inducer from Isodon eriocalyx by a drug discovery platform composed of analytical high-speed counter-current chromatography and the fluorescence-based caspase-3 biosensor detection[J]. Talanta,2010,82(4):1521-1527.
APA Han Q.-B..,Yu T..,Lai F..,Zhou Y..,Feng C..,...&Leung P.-C..(2010).Quick identification of apoptosis inducer from Isodon eriocalyx by a drug discovery platform composed of analytical high-speed counter-current chromatography and the fluorescence-based caspase-3 biosensor detection.Talanta,82(4),1521-1527.
MLA Han Q.-B.,et al."Quick identification of apoptosis inducer from Isodon eriocalyx by a drug discovery platform composed of analytical high-speed counter-current chromatography and the fluorescence-based caspase-3 biosensor detection".Talanta 82.4(2010):1521-1527.
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