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1,3,6,7-Tetrahydroxy-8-prenylxanthone ameliorates inflammatory responses resulting from the paracrine interaction of adipocytes and macrophages
Li, Dan1; Liu, Qianyu1; Sun, Wen1; Chen, Xiuping1; Wang, Ying1; Sun, Yuxiang2; Lin, Ligen1
2018-05
Source PublicationBRITISH JOURNAL OF PHARMACOLOGY
ISSN0007-1188
Volume175Issue:10Pages:1590-1606
Abstract

Background and Purpose

Chronic inflammation in adipose tissue is critical in the onset and development of insulin resistance and type 2 diabetes. Macrophage infiltration into adipose tissue and pro‐inflammatory polarization play key roles in adipose tissue inflammation. The fruit hull of mangosteen (Garcinia mangostana) is used in traditional medicine to treat various inflammatory diseases. However, its role in regulating adipose tissue inflammation is unexplored. This study was designed to identify xanthones from G. mangostana, which could ameliorate adipose tissue inflammation.

Experimental Approach

Expressions of inducible NOS, cytokines, chemokines and components of the NF‐κB and MAPKs pathways were evaluated using Western blotting, immunofluorescence, quantitative real‐time PCR or ELISA. The migration of macrophages towards adipocytes was tested using Transwell experiments in vitro. A murine model of LPS‐induced acute inflammation was used to examine effects of 1,3,6,7‐tetrahydroxy‐8‐prenylxanthone (TPX) on inflammatory responses in adipose tissue in vivo.

Key Results

From a series of xanthones isolated from G. mangostana, TPX was identified as a potent inhibitor of LPS‐induced NO production and IL‐6 secretion in RAW264.7 macrophages. TPX ameliorated LPS‐induced inflammatory responses in RAW264.7 macrophages, and TNF‐α‐mediated inflammation in 3T3‐L1 adipocytes, through inhibiting MAPKs and NF‐κB activation and promoting sirtuin 3 expression. TPX also blocked RAW264.7 macrophages migration towards 3T3‐L1 adipocytes in co‐cultures. Furthermore, TPX alleviated LPS‐induced adipose tissue inflammation in vivo by reducing pro‐inflammatory cytokines and preventing the pro‐inflammatory polarization of macrophages.

Conclusions and Implications

Taken together, our results indicate that TPX disrupts the inflammatory responses between macrophages and adipocytes, and attenuates adipose tissue inflammation.

DOI10.1111/bph.14162
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000430658800003
PublisherWILEY
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLin, Ligen
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau, China
2.Department of Nutrition and Food Science, Texas A&M University, College Station, TX, USA
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Li, Dan,Liu, Qianyu,Sun, Wen,et al. 1,3,6,7-Tetrahydroxy-8-prenylxanthone ameliorates inflammatory responses resulting from the paracrine interaction of adipocytes and macrophages[J]. BRITISH JOURNAL OF PHARMACOLOGY,2018,175(10):1590-1606.
APA Li, Dan.,Liu, Qianyu.,Sun, Wen.,Chen, Xiuping.,Wang, Ying.,...&Lin, Ligen.(2018).1,3,6,7-Tetrahydroxy-8-prenylxanthone ameliorates inflammatory responses resulting from the paracrine interaction of adipocytes and macrophages.BRITISH JOURNAL OF PHARMACOLOGY,175(10),1590-1606.
MLA Li, Dan,et al."1,3,6,7-Tetrahydroxy-8-prenylxanthone ameliorates inflammatory responses resulting from the paracrine interaction of adipocytes and macrophages".BRITISH JOURNAL OF PHARMACOLOGY 175.10(2018):1590-1606.
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