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Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE
Yu Y.1; Li J.1; Chen S.1; Hong Y.1; Ng K.M.1; Luo K.Q.2; Tang B.Z.1
2013-06-12
Source PublicationACS Applied Materials and Interfaces
ISSN19448244 19448252
Volume5Issue:11Pages:4613-4616
Abstract

1-[4-(Bromomethyl)phenyl]-1,2,2-triphenylethene (2) was synthesized and evaluated for specific fluorescent prestaining of proteins containing cysteine (Cys) in SDS-PAGE. The molecule showed classic aggregation-induced emission (AIE) property in protein labeling and its quantum efficiency was further enhanced upon reacting with Cys. The parameters of reaction such as labeling time and concentration of dye and reducing reagent-tris(2-carboxyethyl)phosphine (TCEP) were examined to obtain the optimal labeling condition. In addition to its specific labeling effect, molecule 2 also showed its advantage over traditional self-quenching dyes through labeling Cys containing BSA with different dye/Cys ratios. © 2013 American Chemical Society.

KeywordAggregation-induced Emission Cys Staining Fluorescent Enhancement Tetraphenylethylene Thiol-reactive
DOI10.1021/am401486h
URLView the original
Indexed BySCI
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS IDWOS:000320484000021
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Cited Times [WOS]:9   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Hong Kong University of Science and Technology
2.Nanyang Technological University
Recommended Citation
GB/T 7714
Yu Y.,Li J.,Chen S.,et al. Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE[J]. ACS Applied Materials and Interfaces,2013,5(11):4613-4616.
APA Yu Y..,Li J..,Chen S..,Hong Y..,Ng K.M..,...&Tang B.Z..(2013).Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE.ACS Applied Materials and Interfaces,5(11),4613-4616.
MLA Yu Y.,et al."Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE".ACS Applied Materials and Interfaces 5.11(2013):4613-4616.
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