UM  > 健康科學學院
Characterization of proapoptotic compounds from the bark of Garcinia oblongifolia
Feng C.4; Huang S.-X.5; Gao X.-M.2; Xu H.-X.1; Luo K.Q.3
Source PublicationJournal of Natural Products
ISSN15206025 01633864

Twenty compounds from Garcinia oblongifolia were screened for proapoptotic activity using FRET-based HeLa-C3 sensor cells. Among them, oblongifolins F and G (1 and 2), 1,3,5-trihydroxy-13,13-dimethyl-2H-pyran[7,6-b]xanthone (3), nigrolineaxanthone T (4), and garcicowin B (5) showed significant proapoptotic activity at a concentration of 10 μM. Bioassessments were then performed to evaluate the potential of these compounds for therapeutic application. All five compounds showed significant cytotoxicity and caspase-3-activating ability in cervical cancer HeLa cells, with compounds 1 and 2 having the highest potencies. All five compounds specifically induced caspase-dependent apoptosis, which could be prevented by the pan-caspase inhibitor zVAD-fmk. In particular, 3 induced apoptosis through mitotic arrest. Compounds 1-5 displayed similar IC values (3.9-16.5 μM) against the three cancer cell lines HeLa, MDA-MB-435, and HepG2. In addition, compounds 1, 2, and 4 exhibited similar and potent IC values (2.4-5.1 μM) against several breast and colon cancer cell lines, including those overexpressing either HER2 or P-glycoprotein. HER2 and P-glycoprotein are known factors that confer resistance to anticancer drugs in cancer cells. This is the first study on the cytotoxicity, caspase-3-activing ability, and specificity of proapoptotic compounds isolated from G. oblongifolia in HeLa cells. The potential application of these compounds against HER2- or P-glycoprotein-overexpressing cancer cells was investigated. © 2014 The American Chemical Society and American Society of Pharmacognosy.

URLView the original
Indexed BySCI
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS IDWOS:000336510700003
Fulltext Access
Citation statistics
Cited Times [WOS]:11   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Shanghai University of Traditional Chinese Medicine
2.Yunnan Minzu University
3.Nanyang Technological University
4.Hong Kong University of Science and Technology
5.Kunming Institute of Botany Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Feng C.,Huang S.-X.,Gao X.-M.,et al. Characterization of proapoptotic compounds from the bark of Garcinia oblongifolia[J]. Journal of Natural Products,2014,77(5):1111-1116.
APA Feng C.,Huang S.-X.,Gao X.-M.,Xu H.-X.,&Luo K.Q..(2014).Characterization of proapoptotic compounds from the bark of Garcinia oblongifolia.Journal of Natural Products,77(5),1111-1116.
MLA Feng C.,et al."Characterization of proapoptotic compounds from the bark of Garcinia oblongifolia".Journal of Natural Products 77.5(2014):1111-1116.
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Feng C.]'s Articles
[Huang S.-X.]'s Articles
[Gao X.-M.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Feng C.]'s Articles
[Huang S.-X.]'s Articles
[Gao X.-M.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Feng C.]'s Articles
[Huang S.-X.]'s Articles
[Gao X.-M.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.