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Expression of Smad4 Gene in Pichia pastoris and Identification of the Protein
Liu Y.-P.1; Yang X.3; Xu X.-L.2; Deng J.-X.3; Cheng X.3; Huang C.-F.3
2000-12-01
Source PublicationActa Biochimica et Biophysica Sinica
ISSN05829879
Volume32Issue:3
AbstractSmad4 is a novel tumor suppressor gene which is mutated or deleted in about 50% of pancreatic carcinoma and 30% of colon cancer. SMAD4 was expressed in Pichia pastoris and was characterized. The molecular weight of the expression product was shown to be about 67 kD, and 13 amino acids in N terminal were determined and were identical with the putative sequence from Smad4 cDNA, and it was able to specifically react with the antibody against SMAD4.
KeywordPichia pastoris Smad4 Yeast expression
URLView the original
Language英語
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Document TypeJournal article
专题University of Macau
Affiliation1.Military Medical Institute of Guangzhou
2.National Institute of Diabetes and Digestive and Kidney Diseases
3.Academy of Military Medical Science China
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GB/T 7714
Liu Y.-P.,Yang X.,Xu X.-L.,et al. Expression of Smad4 Gene in Pichia pastoris and Identification of the Protein[J]. Acta Biochimica et Biophysica Sinica,2000,32(3).
APA Liu Y.-P.,Yang X.,Xu X.-L.,Deng J.-X.,Cheng X.,&Huang C.-F..(2000).Expression of Smad4 Gene in Pichia pastoris and Identification of the Protein.Acta Biochimica et Biophysica Sinica,32(3).
MLA Liu Y.-P.,et al."Expression of Smad4 Gene in Pichia pastoris and Identification of the Protein".Acta Biochimica et Biophysica Sinica 32.3(2000).
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