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Bright polymer dots tracking stem cell engraftment and migration to injured mouse liver
Chen D.1; Li Q.1; Meng Z.1; Guo L.1; Tang Y.1; Liu Z.1; Yin S.1; Qin W.1; Yuan Z.2; Zhang X.2; Wu C.1
2017
Source PublicationTheranostics
ISSN18387640
Volume7Issue:7Pages:1820-1834
Abstract

Stem cell therapy holds promise for treatment of intractable diseases and injured organs. For clinical translation, it is pivotal to understand the homing, engraftment, and differentiation processes of stem cells in a living body. Here we report near-infrared (NIR) fluorescent semiconductor polymer dots (Pdots) for bright labeling and tracking of human mesenchymal stem cells (MSCs). The Pdots exhibit narrow-band emission at 775 nm with a quantum yield of 22%, among the highest value for various NIR probes. The Pdots together with a cell penetrating peptide are able to track stem cells over two weeks without disturbing their multipotent properties, as confirmed by the analyses on cell proliferation, differentiation, stem-cell markers, and immunophenotyping. The in vivo cell tracking was demonstrated in a liver-resection mouse model, which indicated that the Pdot-labeled MSCs after tail-vein transplantation were initially trapped in lung, gradually migrated to the injured liver, and then proliferated into cell clusters. Liver-function analysis and histological examination revealed that the inflammation induced by liver resection was apparently decreased after stem cell transplantation. With the bright labeling, superior biocompatibility, and long-term tracking performance, the Pdot probes are promising for stem cell research and regenerative medicine.

KeywordCell Tracking In Vivo imagIng Near-infrared Fluorescence Semiconductor Polymer Dot Stem Cells
DOI10.7150/thno.18614
URLView the original
Indexed BySCI
Language英语
WOS Research AreaResearch & Experimental Medicine
WOS SubjectMedicine, Research & Experimental
WOS IDWOS:000402093200004
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Cited Times [WOS]:15   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Jilin University
2.Universidade de Macau
Recommended Citation
GB/T 7714
Chen D.,Li Q.,Meng Z.,et al. Bright polymer dots tracking stem cell engraftment and migration to injured mouse liver[J]. Theranostics,2017,7(7):1820-1834.
APA Chen D..,Li Q..,Meng Z..,Guo L..,Tang Y..,...&Wu C..(2017).Bright polymer dots tracking stem cell engraftment and migration to injured mouse liver.Theranostics,7(7),1820-1834.
MLA Chen D.,et al."Bright polymer dots tracking stem cell engraftment and migration to injured mouse liver".Theranostics 7.7(2017):1820-1834.
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