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CRISPR-Cas9: from Genome Editing to Cancer Research
Si Chen; Heng Sun; Kai Miao; Chu-Xia Deng
2016
Source PublicationInternational Journal of Biological Sciences
ISSN1449-2288
Volume12Issue:12Pages:1427-1436
Abstract

Cancer development is a multistep process triggered by innate and acquired mutations, which cause the functional abnormality and determine the initiation and progression of tumorigenesis. Gene editing is a widely used engineering tool for generating mutations that enhance tumorigenesis. The recent developed clustered regularly interspaced short palindromic repeats-CRISPR-associated 9 (CRISPR-Cas9) system renews the genome editing approach into a more convenient and efficient way. By rapidly introducing genetic modifications in cell lines, organs and animals, CRISPR-Cas9 system extends the gene editing into whole genome screening, both in loss-of-function and gain-of-function manners. Meanwhile, the system accelerates the establishment of animal cancer models, promoting in vivo studies for cancer research. Furthermore, CRISPR-Cas9 system is modified into diverse innovative tools for observing the dynamic bioprocesses in cancer studies, such as image tracing for targeted DNA, regulation of transcription activation or repression. Here, we view recent technical advances in the application of CRISPR-Cas9 system in cancer genetics, large-scale cancer driver gene hunting, animal cancer modeling and functional studies.

KeywordCrispr Genome Editing Large-scale Screening Animal Model Cancer
DOIhttp://doi.org/10.7150/ijbs.17421
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS SubjectBiochemistry & Molecular Biology ; Biology
WOS IDWOS:000396425200003
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Cited Times [WOS]:14   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Co-First AuthorSi Chen; Heng Sun
Corresponding AuthorChu-Xia Deng
AffiliationFaculty of Health Sciences, University of Macau, Macau SAR, China
First Author AffilicationFaculty of Health Sciences
Corresponding Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Si Chen,Heng Sun,Kai Miao,et al. CRISPR-Cas9: from Genome Editing to Cancer Research[J]. International Journal of Biological Sciences,2016,12(12):1427-1436.
APA Si Chen,Heng Sun,Kai Miao,&Chu-Xia Deng.(2016).CRISPR-Cas9: from Genome Editing to Cancer Research.International Journal of Biological Sciences,12(12),1427-1436.
MLA Si Chen,et al."CRISPR-Cas9: from Genome Editing to Cancer Research".International Journal of Biological Sciences 12.12(2016):1427-1436.
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