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Purification, crystallization, and preliminary X-ray studies of a bifunctional 5,10-methenyl/methylene tetrahydrofolate cyclohydrolase/dehydrogenase from Escherichia coli
Cheung E.2; D'Ari L.2; Rabinowitz J.C.2; Dyer D.H.1; Huang J.-Y.1; Stoddard B.L.1
1997-03-22
Source PublicationProteins: Structure, Function and Genetics
ISSN08873585
Volume27Issue:2Pages:322-324
AbstractA bifunctional enzyme that catalyzes the conversion of formyltetrahydrofolate to methylene-tetrahydrofolate (5,10- methenyltetrahydrofolate cyclohydrolase and 5,10-methylene tetrahydrofolate dehydrogenase), has been subcloned from a cDNA library, purified to homogeneity, and crystallized. The crystals belong to space group 1222, with unit cell dimensions of a = 64.5 Å, b = 84.9 Å, c = 146.1 Å. The crystal unit cell and diffraction is consistent with an asymmetric unit consisting of the enzyme monomer, and a specific volume of the unit cell of 3.2 Å/Da. The crystals diffract to at least 2.8 Å resolution after flash-cooling, when using a rotating anode x-ray source and an RAXIS image plate detector. A 2.56 Å resolution native data set has been collected at beamline X12-C at the NSLS.
Keywordfolate coenzymes protein crystallization purine synthesis tetrahydrofolate
DOI10.1002/(SICI)1097-0134(199702)27:2<322::AID-PROT19>3.0.CO;2-O
URLView the original
Language英語
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Document TypeJournal article
专题University of Macau
Affiliation1.Fred Hutchinson Cancer Research Center
2.University of California, Berkeley
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Cheung E.,D'Ari L.,Rabinowitz J.C.,et al. Purification, crystallization, and preliminary X-ray studies of a bifunctional 5,10-methenyl/methylene tetrahydrofolate cyclohydrolase/dehydrogenase from Escherichia coli[J]. Proteins: Structure, Function and Genetics,1997,27(2):322-324.
APA Cheung E.,D'Ari L.,Rabinowitz J.C.,Dyer D.H.,Huang J.-Y.,&Stoddard B.L..(1997).Purification, crystallization, and preliminary X-ray studies of a bifunctional 5,10-methenyl/methylene tetrahydrofolate cyclohydrolase/dehydrogenase from Escherichia coli.Proteins: Structure, Function and Genetics,27(2),322-324.
MLA Cheung E.,et al."Purification, crystallization, and preliminary X-ray studies of a bifunctional 5,10-methenyl/methylene tetrahydrofolate cyclohydrolase/dehydrogenase from Escherichia coli".Proteins: Structure, Function and Genetics 27.2(1997):322-324.
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