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Studying Protein-DNA Complexes Using Gold Nanoparticles by Exploiting Particle Aggregation, Refractive Index Change, and Fluorescence Quenching and Enhancement Principles
Sutarlie L.4; Aung K.M.M.4; Lim M.G.L.3; Lukman S.4; Cheung E.3; Su X.4
2014
Source PublicationPlasmonics
ISSN15571963 15571955
Volume9Issue:4Pages:753-763
Abstract

Gold nanoparticles (AuNPs) have a unique optical phenomena termed localized surface plasmon resonance that is determined by particle shape, size, interparticle distance (or aggregation status), and local refractive index. AuNPs can also modulate fluorescence emission of proximal fluorophores under the Förster resonance energy transfer and/or nanoparticle surface energy transfer mechanisms. In this study, we use AuNPs (13 and 100 nm in diameter) as sensing elements to study sequence-dependent protein-DNA interactions by exploring all possible principles, namely (1) particle aggregation based colorimetric sensing, (2) refractive index sensing, and (3) fluorescence quenching/enhancement based fluorimetric sensing, exemplified by transcription factors, i.e., FoxA1 and AP2γ, and their respective DNAs. We conclude that the first principle, i.e., particle aggregation-based colorimetric assay that measures preformed complex by exploring complex protection of AuNPs from salt-induced aggregation, is simple to use. However, its performance is protein specific. For second and third principles that measure on-particle complex formation, we prove that the fluorescence quenching/enhancement assays supported by AuNPs are more sensitive than assays exploiting analyte-binding induced refractive index principle. This study provides a comprehensive assessment of the versatility of AuNPs as sensing probes for studying bioaffinity interactions especially protein-DNA complexes. The discovery of the DNA binding properties of FoxA1 and AP-2γ is important in revealing their roles in gene regulation. © 2014 Springer Science+Business Media New York.

KeywordBiosensors Fluorescence Enhancement Fluorescence Quenching Gold Nanoparticles Protein-dna Complexes Transcription Factors
DOI10.1007/s11468-013-9655-2
URLView the original
Indexed BySCI
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science
WOS SubjectChemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS IDWOS:000341423800005
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Cited Times [WOS]:9   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Nanyang Technological University
2.Yong Loo Lin School of Medicine
3.A-Star, Genome Institute of Singapore
4.A-Star, Institute of Materials Research and Engineering
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Sutarlie L.,Aung K.M.M.,Lim M.G.L.,et al. Studying Protein-DNA Complexes Using Gold Nanoparticles by Exploiting Particle Aggregation, Refractive Index Change, and Fluorescence Quenching and Enhancement Principles[J]. Plasmonics,2014,9(4):753-763.
APA Sutarlie L.,Aung K.M.M.,Lim M.G.L.,Lukman S.,Cheung E.,&Su X..(2014).Studying Protein-DNA Complexes Using Gold Nanoparticles by Exploiting Particle Aggregation, Refractive Index Change, and Fluorescence Quenching and Enhancement Principles.Plasmonics,9(4),753-763.
MLA Sutarlie L.,et al."Studying Protein-DNA Complexes Using Gold Nanoparticles by Exploiting Particle Aggregation, Refractive Index Change, and Fluorescence Quenching and Enhancement Principles".Plasmonics 9.4(2014):753-763.
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