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The suppression of medium acidosis improves the maintenance and differentiation of human pluripotent stem cells at high density in defined cell culture medium
Liu W.1; Ren Z.1; Lu K.1; Song C.1; Cheung E.C.W.1; Zhou Z.2; Chen G.1
2018-04-05
Source PublicationInternational Journal of Biological Sciences
ISSN14492288
Volume14Issue:5Pages:485-496
Abstract

Cell density has profound impacts on the cell culture practices of human pluripotent stem cells. The regulation of cell growth, cell death, pluripotency and differentiation converge at high density, but it is largely unknown how different regulatory mechanisms act at this stage. We use a chemically defined medium to systemically examine cellular activities and the impact of medium components in high-density culture. We show that medium acidosis is the main factor that alters cell cycle, gene expression and cellular metabolism at high cell density. The low medium pH leads to inhibition of glucose consumption, cell cycle arrest, and subsequent cell death. At high cell density, the suppression of medium acidosis with sodium bicarbonate (NaHCO) significantly increases culture capacity for stem cell survival, derivation, maintenance and differentiation. Our study provides a simple and effective tool to improve stem cell maintenance and applications.

KeywordAcidosis Cell Death Differentiation Glycolysis Human Pluripotent Stem Cells
DOIhttp://doi.org/10.7150/ijbs.24681
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS SubjectBiochemistry & Molecular Biology ; Biology
WOS IDWOS:000430149900001
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被引频次[WOS]:2   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题Faculty of Health Sciences
Affiliation1.Universidade de Macau
2.Fuwai Hospital
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Liu W.,Ren Z.,Lu K.,et al. The suppression of medium acidosis improves the maintenance and differentiation of human pluripotent stem cells at high density in defined cell culture medium[J]. International Journal of Biological Sciences,2018,14(5):485-496.
APA Liu W..,Ren Z..,Lu K..,Song C..,Cheung E.C.W..,...&Chen G..(2018).The suppression of medium acidosis improves the maintenance and differentiation of human pluripotent stem cells at high density in defined cell culture medium.International Journal of Biological Sciences,14(5),485-496.
MLA Liu W.,et al."The suppression of medium acidosis improves the maintenance and differentiation of human pluripotent stem cells at high density in defined cell culture medium".International Journal of Biological Sciences 14.5(2018):485-496.
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