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A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells
Zhizhen Chen1; Jie Liu1; Dafeng Chu2; Yaming Shan3; Guixing Ma4; Hongmin Zhang4; Xiaohua Douglas Zhang1; Pu Wang5; Qiang Chen1; Chuxia Deng1; Weizao Chen6; Dimiter Dimitrov7; Qi Zhao1
2018
Source PublicationInternational Journal of Biological Sciences
ISSN1449-2288
Volume14Issue:7Pages:799–806
Abstract

The insulin-like growth factors (IGFs), IGF-I and IGF-II, are essential for regulating cell growth, differentiation and metastasis of a broad range of malignancies. The IGF-I/II actions are mediated through the IGF receptor type 1 (IGF-1R) and the insulin receptor (IR), which are overexpressed in multiple types of tumors. Here, we have firstly identified a human engineered antibody domain (eAd) from a phage-displayed VH library. The eAd suppressed the signal transduction of IGF-1R mediated by exogenous IGF-I or IGF-II in breast cancer cell lines through neutralizing both IGF-I and IGF-II. It also significantly inhibited the growth of breast cancer cells. Therefore, the anti-IGF-I/II eAd offers an alternative approach to target both the IGF-1R signaling pathways through the inhibition of IGF-I/II.

KeywordInsulin-like Growth Factor Yeast Display Affinity Maturation Ead
DOIhttp://dx.doi.org/10.7150/ijbs.25928
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS SubjectBiochemistry & Molecular Biology ; Biology
WOS IDWOS:000433261800011
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Co-First AuthorZhizhen Chen; Jie Liu
Affiliation1.Faculty of Health Sciences, University of Macau, Macau, China
2.Department of Bioengineering, University of California, Los Angeles, California, USA.
3.National Engineering Laboratory for AIDS Vaccine, School of Life Science, Jilin University, Changchun, Jilin, China
4.Department of Biology, Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research, Shenzhen Key Laboratory of Cell Microenvironment, SUSTech-HKU joint laboratories for matrix biology and diseases, Southern University of Science and Technology, Shenzhen, Guangdong, China
5.Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Guangdong, China
6.Center for Cancer Research, National Cancer Institute-Frederick, National Institutes of Health, Maryland, USA
7.Center for Antibody Therapeutics, University of Pittsburgh Medical School, Pennsylvania, USA
First Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Zhizhen Chen,Jie Liu,Dafeng Chu,et al. A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells[J]. International Journal of Biological Sciences,2018,14(7):799–806.
APA Zhizhen Chen.,Jie Liu.,Dafeng Chu.,Yaming Shan.,Guixing Ma.,...&Qi Zhao.(2018).A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells.International Journal of Biological Sciences,14(7),799–806.
MLA Zhizhen Chen,et al."A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells".International Journal of Biological Sciences 14.7(2018):799–806.
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