UM
Highly efficient ratiometric extracellular oxygen sensors through physical incorporation of a conjugated polymer and PtTFPP in graft copolymers
Zhao, Qian; Pan, Tingting; Xiang, Gang; Mei, Zhipeng; Jiang, Jiapei; Li, Gang; Zou, Xianshao; Chen, Meiwan; Sun, Dazhi; Jiang, Shimei; Tian, Yanqing
2018-11-10
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
Volume273Pages:242-252
AbstractAn amphiphilic graft copolymer (P3) composed of poly(oligo (ethylene glycol) methacrylate) (POEGMA) as a hydrophilic segment and a polystyrene (PS) as a hydrophobic chain was prepared. P3 was used to incorporate a highly efficient hydrophobic oxygen probe platinum(II)-5,10,15,20-tetrakis-(2,3,4,5,6-pentafluorophenyl)-porphyrin (PtTFPP) in its micelles to enable the application of PtTFPP in aqueous solution for biosensing. For achieving ratiometric oxygen sensing, a hydrophobic conjugated polymer (CP) was used as a fluorescence resonance energy transfer (FRET) donor for PtTFPP-the FRET acceptor. Results showed that diameters of these nano-oxygen sensors ranging from 50 to 60 nm and FRET efficiency as high as 98% could be achieved. Further, FRET contributed to the brightness of PtTFPP in micelles. High quantum yields of PtTFPP in non-FRET micelles and FRET micelles under nitrogen were achieved to be 0.109 and 0.231, respectively. The sensors were found to be able to measure oxygen concentrations from 0.082 to 40.9 mg/L at room temperature with linear Stern-Volmer constants. These sensors were also demonstrated to be suitable for monitoring extracellular oxygen consumption of E. Coll and HeLa cells. This study illustrated a feasible approach to achieve highly efficient ratiometric oxygen sensors without specific structure modification of the efficient PtTFPP probe, however using an amphiphilic graft copolymer approach.
KeywordAmphiphilic graft copolymer Conjugated polymer Oxygen sensor FRET Micelles Oxygen consumption
DOI10.1016/j.snb.2018.06.026
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:000441519000032
PublisherELSEVIER SCIENCE SA
The Source to ArticleWOS
Fulltext Access
Citation statistics
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Zhao, Qian,Pan, Tingting,Xiang, Gang,et al. Highly efficient ratiometric extracellular oxygen sensors through physical incorporation of a conjugated polymer and PtTFPP in graft copolymers[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,273:242-252.
APA Zhao, Qian.,Pan, Tingting.,Xiang, Gang.,Mei, Zhipeng.,Jiang, Jiapei.,...&Tian, Yanqing.(2018).Highly efficient ratiometric extracellular oxygen sensors through physical incorporation of a conjugated polymer and PtTFPP in graft copolymers.SENSORS AND ACTUATORS B-CHEMICAL,273,242-252.
MLA Zhao, Qian,et al."Highly efficient ratiometric extracellular oxygen sensors through physical incorporation of a conjugated polymer and PtTFPP in graft copolymers".SENSORS AND ACTUATORS B-CHEMICAL 273(2018):242-252.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhao, Qian]'s Articles
[Pan, Tingting]'s Articles
[Xiang, Gang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhao, Qian]'s Articles
[Pan, Tingting]'s Articles
[Xiang, Gang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhao, Qian]'s Articles
[Pan, Tingting]'s Articles
[Xiang, Gang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.