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Generation of Smurf2 conditional knockout mice
Tang Y.1; Tang L.-Y.1; Xu X.1; Li C.2; Deng C.2; Zhang Y.E.1
Source PublicationInternational Journal of Biological Sciences

Smad ubiquitin regulatory factor 2 (Smurf2) is a HECT domain-containing E3 ubiquitin ligase. Together with its closely related homolog Smurf1, Smurf2 was initially recognized as a negative regulator of transforming growth factor-β (TGF-β) and bone morphogenetic protein (BMP) signaling, but subsequent studies have expanded its function to regulate many different signaling pathways and play important roles in genomic stability, cell polarity, tissue homeostasis and carcinogenesis. Mice with conventional knockout of Smurf1 or Smurf2 alleles are viable, but conventional Smurf1 and Smurf2 double knockout mice were early embryonic lethal. In order to study the physiological function of Smurfs during late stage of embryonic development or in adult animals, we generated Smurf2 mice carrying a targeted mutation for conditional Smurf2 gene inactivation. We demonstrated that Cre-mediated recombination using Alb-Cre, a Cre line expressed in hepatocyte, results in specific deletion of the gene in liver tissue. We also showed that Cre-mediated recombination in mouse embryonic fibroblasts (MEFs) with Smurf2 genotype resulted in generation of Smurf2 knockout MEFs, and Smurf2 deficiency affects multiple signaling pathways. Therefore, this animal model will be useful to study the distinct roles of Smurf2 in different tissues at different ages.

KeywordKnockout Liver Loxp Mice Smurf2
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Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS SubjectBiochemistry & Molecular Biology ; Biology
WOS IDWOS:000430149900006
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Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorZhang Y.E.
Affiliation1.National Cancer Institute
2.National Institute of Diabetes and Digestive and Kidney Diseases
3.Universidade de Macau
Recommended Citation
GB/T 7714
Tang Y.,Tang L.-Y.,Xu X.,et al. Generation of Smurf2 conditional knockout mice[J]. International Journal of Biological Sciences,2018,14(5):542-548.
APA Tang Y.,Tang L.-Y.,Xu X.,Li C.,Deng C.,&Zhang Y.E..(2018).Generation of Smurf2 conditional knockout mice.International Journal of Biological Sciences,14(5),542-548.
MLA Tang Y.,et al."Generation of Smurf2 conditional knockout mice".International Journal of Biological Sciences 14.5(2018):542-548.
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