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Characterization of active antiplatelet chemical compositions of edible Citrus limon through ultraperformance liquid chromatography single quadrupole mass spectrometry-based chemometrics
Zhang, Qian; Tan, Chengning; Cai, Liang; Xia, Fangbo; Gao, Die; Yang, Fengqing; Chen, Hua; Xia, Zhining
2018-05
Source PublicationFOOD & FUNCTION
ISSN2042-6496
Volume9Issue:5Pages:2762-2773
AbstractCitrus limon L. (lemon, family: Rutaceae) is the third most popular edible fruit among the Citrus species. Our previous study has shown the significant antiplatelet activity of lemon extracts. The aim of the present study is to identify the features (retention time, m/z) associated with the antiplatelet activity of lemons by correlating a platelet aggregation assay with ultra-performance liquid chromatography single quadrupole mass spectrometry-based chemometrics analysis. The primary bioactivity-guided test results revealed that the butanol (BA) and ethyl acetate (EA) liquid-liquid extraction sections of the ethanol extract of lemons had significant inhibitory effects on platelet aggregation. Upon further separating the combined BA and EA sections with a silica column, four different active fractions were obtained, and their LC-MS data were collected. After modeling by two multivariate statistical techniques, namely, principal component analysis and orthogonal partial least squares discriminate analysis seven markers were predicted, identified, and tentatively classified as priority markers of bioactivity in lemons. Among them, the antiplatelet activity of four marker compounds, namely, oxypeucedanin hydrate, citric acid, diosmin, and limetin at concentrations lower than 300 mu M was confirmed. Moreover, the specific mechanism of limetin interaction with the TP ss receptor of thromboxane A2 and the effect of limetin on the PI3 K/Rap-1b signaling pathway through the ss. subunit of GPCR (i) in platelet aggregation were studied by differential proteomic analysis to illustrate the validity and persistence of these markers for application in lemon fruit platforms.
DOI10.1039/c8fo00403j
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Food Science & Technology
WOS SubjectBiochemistry & Molecular Biology ; Food Science & Technology
WOS IDWOS:000433198000020
PublisherROYAL SOC CHEMISTRY
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Zhang, Qian,Tan, Chengning,Cai, Liang,et al. Characterization of active antiplatelet chemical compositions of edible Citrus limon through ultraperformance liquid chromatography single quadrupole mass spectrometry-based chemometrics[J]. FOOD & FUNCTION,2018,9(5):2762-2773.
APA Zhang, Qian.,Tan, Chengning.,Cai, Liang.,Xia, Fangbo.,Gao, Die.,...&Xia, Zhining.(2018).Characterization of active antiplatelet chemical compositions of edible Citrus limon through ultraperformance liquid chromatography single quadrupole mass spectrometry-based chemometrics.FOOD & FUNCTION,9(5),2762-2773.
MLA Zhang, Qian,et al."Characterization of active antiplatelet chemical compositions of edible Citrus limon through ultraperformance liquid chromatography single quadrupole mass spectrometry-based chemometrics".FOOD & FUNCTION 9.5(2018):2762-2773.
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