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Roles of Figla/figla in juvenile ovary development and follicle formation during zebrafish gonadogenesis
Mingming Qin1; Zhiwei Zhang1; Weiyi Song1; Queenie Wing-Lei Wong1; Weiting Chen1; Niranjan Shirgaonkar2; Wei Ge1
2018-08-31
Source PublicationEndocrinology
ISSN1945-7170
Volume159Issue:11Pages:3699–3722
Abstract

Sex determination and differentiation are complex processes. As a juvenile hermaphrodite or undifferentiated gonochorist, zebrafish undergo a special juvenile ovarian phase during sex differentiation, making it an excellent model for studying early oogenesis and folliculogenesis. We provide lines of evidence at morphological, molecular, and genetic levels for roles of factor in the germline α(Figla), an oocyte-specific transcription factor, in early zebrafish gonadogenesis. As in mammals, Figla/figla was also expressed in the gonads and its expression in the ovary was also restricted to early oocytes. Disruption of figla gene by CRISPR/Cas9 led to an all-male phenotype in the mutant. Detailed analysis of early gonadal development showed that the germ cells in the mutant were clustered in cysts and underwent meiosis, forming oocytes at prefollicular chromatin nucleolar (CN) stage (stage IA). However, the subsequent transition from cystic CN oocytes to individual follicular perinucleolar oocytes (stage IB) was blocked, resulting in an all-male phenotype in the mutant. The phenotype of figla mutant could not be rescued by estrogen treatment, in contrast to cyp19a1amutant, and introduction of tp53 mutation also had no effect, unlike in fancd1 and fancl mutants. Transcriptome analysis revealed that many biological processes and pathways related to germ cell development, especially oogenesis, were upregulated in the presence of Figla and that the regulation of figla expression may involve heat shock proteins. Our results strongly suggest important roles for Figla in juvenile ovary development, especially the formation of individual follicles from cystic oocytes.

DOIhttps://doi.org/10.1210/en.2018-00648
Indexed BySCI
Language英语
WOS Research AreaEndocrinology & Metabolism
WOS SubjectEndocrinology & Metabolism
WOS IDWOS:000453355700011
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorWei Ge
Affiliation1.Center of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences, University of Macau 999078, Taipa, Macau, China
2.Genomics and Bioinformatics Core, Faculty of Health Sciences, University of Macau, Taipa, Macau 999078, China
First Author AffilicationFaculty of Health Sciences
Corresponding Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Mingming Qin,Zhiwei Zhang,Weiyi Song,et al. Roles of Figla/figla in juvenile ovary development and follicle formation during zebrafish gonadogenesis[J]. Endocrinology,2018,159(11):3699–3722.
APA Mingming Qin.,Zhiwei Zhang.,Weiyi Song.,Queenie Wing-Lei Wong.,Weiting Chen.,...&Wei Ge.(2018).Roles of Figla/figla in juvenile ovary development and follicle formation during zebrafish gonadogenesis.Endocrinology,159(11),3699–3722.
MLA Mingming Qin,et al."Roles of Figla/figla in juvenile ovary development and follicle formation during zebrafish gonadogenesis".Endocrinology 159.11(2018):3699–3722.
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