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Disruption of Zebrafish Follicle-Stimulating Hormone Receptor (fshr) But Not Luteinizing Hormone Receptor (lhcgr) Gene by TALEN Leads to Failed Follicle Activation in Females Followed by Sexual Reversal to Males
Zhang, Zhiwei1,2; Lau, Shuk-Wa2; Zhang, Lingling2; Ge, Wei1,2
2015-10
Source PublicationENDOCRINOLOGY
ISSN0013-7227
Volume156Issue:10Pages:3747-3762
Abstract

Gonadotropins are primary hormones that control vertebrate reproduction. In a recent study, we analyzed the impacts of FSH and LH on zebrafish reproduction by disrupting FSH and LH-beta genes (fshb and lhb) using transcription activator-like effector nuclease (TALEN) technology. Using the same approach, we successfully deleted FSH and LH receptor genes (fshr and lhcgr) in the present study. In contrast to the deficiency of its cognate ligand FSH, the fshr-deficient females showed a complete failure of follicle activation with all ovarian follicles arrested at the primary growth-previtellogenic transition, which is the marker for puberty onset in females. Interestingly, after blockade at the primary growth stage for varying times, all females reversed to males, and all these males were fertile. In fshr-deficient males, spermatogenesis was normal in adults, but the initiation of spermatogenesis in juveniles was retarded. In contrast to fshr, the deletion of the lhcgr gene alone caused no obvious phenotypes in both males and females; however, double mutation of fshr and lhcgr resulted in infertile males. In summary, our results in the present study showed that Fshr was indispensable to folliculogenesis and the disruption of the fshr gene resulted in a complete failure of follicle activation followed by masculinization into males. In contrast, lhcgr does not seem to be essential to zebrafish reproduction in both males and females. Neither Fshr nor Lhcgr deficiency could phenocopy the deficiency of their cognate ligands FSH and LH, which is likely due to the fact that Fshr can be activated by both FSH and LH in the zebrafish.

DOI10.1210/en.2015-1039
URLView the original
Indexed BySCI
Language英语
WOS Research AreaEndocrinology & Metabolism
WOS SubjectEndocrinology & Metabolism
WOS IDWOS:000362235300040
PublisherENDOCRINE SOC
Fulltext Access
Citation statistics
Cited Times [WOS]:53   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Centre of Reproduction, Development and Aging, Faculty of Health Sciences, University ofMacau, Taipa, Macau China;
2.School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
First Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Zhang, Zhiwei,Lau, Shuk-Wa,Zhang, Lingling,et al. Disruption of Zebrafish Follicle-Stimulating Hormone Receptor (fshr) But Not Luteinizing Hormone Receptor (lhcgr) Gene by TALEN Leads to Failed Follicle Activation in Females Followed by Sexual Reversal to Males[J]. ENDOCRINOLOGY,2015,156(10):3747-3762.
APA Zhang, Zhiwei,Lau, Shuk-Wa,Zhang, Lingling,&Ge, Wei.(2015).Disruption of Zebrafish Follicle-Stimulating Hormone Receptor (fshr) But Not Luteinizing Hormone Receptor (lhcgr) Gene by TALEN Leads to Failed Follicle Activation in Females Followed by Sexual Reversal to Males.ENDOCRINOLOGY,156(10),3747-3762.
MLA Zhang, Zhiwei,et al."Disruption of Zebrafish Follicle-Stimulating Hormone Receptor (fshr) But Not Luteinizing Hormone Receptor (lhcgr) Gene by TALEN Leads to Failed Follicle Activation in Females Followed by Sexual Reversal to Males".ENDOCRINOLOGY 156.10(2015):3747-3762.
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