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Expression and functional characterization of intrafollicular GH-IGF system in the zebrafish ovary
Zhou, Rui2; Yu, Susana Man Ying2; Ge, Wei1,2
2016-06
Source PublicationGENERAL AND COMPARATIVE ENDOCRINOLOGY
ISSN0016-6480
Volume232Pages:32-42
Abstract

The somatotrophic axis plays important roles in influencing reproduction. All key members of this axis including growth hormone (GH, gh), GH receptors (ghra and ghrb), insulin-like growth factors (IGFs, igf1, igf2 and igf3) and IGF receptors (igf1ra and igf1rb) were detected in the zebrafish ovary. GH was exclusively expressed in the full-grown oocytes, while its receptors were detectable in both the follicle cells and oocytes. The IGFs and their receptors were all expressed in both compartments except igf3, which was expressed in the follicle cells only. During folliculogenesis, there was a sharp decrease of gh expression at follicle activation; however, the expression of its receptors increased significantly. The expression profiles of igf1, igf2a, and igf2b were similar to that of fshr, whereas igf3 expression was close to lhcgr, suggesting differential roles for different forms of IGFs in follicle development. To examine if the ovarian GH-IGF system is regulated by gonadotropins (e.g., hCG) and GH, we performed in vitro experiments using cultured zebrafish follicle cells. The expression of igf1 and igf1ra, but not others, was down regulated by hCG (LH analog), whereas recombinant zebrafish GH stimulated igf1 expression. In addition, GH also increased the expression of activin beta A subunit (inhbaa). In agreement with this, the stimulatory effect of GH but not IGF-I on oocyte maturation could be abolished by follistatin. In conclusion, the present study revealed an intrafollicular network involving GH-IGF mini-axis in the zebrafish ovary; however, it might not work in the same way as that of the systemic somatotrophic axis. (C) 2015 Elsevier Inc. All rights reserved.

KeywordGrowth Hormone (Gh) Insulin-like Growth Factors (Igfs) Ovary Follicles Zebrafish
DOI10.1016/j.ygcen.2015.11.018
URLView the original
Indexed BySCI
Language英语
WOS Research AreaEndocrinology & Metabolism
WOS SubjectEndocrinology & Metabolism
WOS IDWOS:000378187600005
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Fulltext Access
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Cited Times [WOS]:13   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorGe, Wei
Affiliation1.Centre of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, Taipa, Macau, China
2.School of Life Sciences and Centre for Cell and Developmental Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
Corresponding Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Zhou, Rui,Yu, Susana Man Ying,Ge, Wei. Expression and functional characterization of intrafollicular GH-IGF system in the zebrafish ovary[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2016,232:32-42.
APA Zhou, Rui,Yu, Susana Man Ying,&Ge, Wei.(2016).Expression and functional characterization of intrafollicular GH-IGF system in the zebrafish ovary.GENERAL AND COMPARATIVE ENDOCRINOLOGY,232,32-42.
MLA Zhou, Rui,et al."Expression and functional characterization of intrafollicular GH-IGF system in the zebrafish ovary".GENERAL AND COMPARATIVE ENDOCRINOLOGY 232(2016):32-42.
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