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Whole-body multispectral photoacoustic imaging of adult zebrafish
Huang, Na1,4; Gu, Heng1,4; Qi, Weizhi1,4; Zhang, Zhiwei3; Rong, Jian1,2; Yuan, Zhen3; Ge, Wei3; Jiang, Huabei1,2; Xi, Lei1,2
2016-09
Source PublicationBIOMEDICAL OPTICS EXPRESS
ISSN2156-7085
Volume7Issue:9Pages:3543-3550
Abstract

The zebrafish, an ideal vertebrate for studying developmental biology and genetics, is increasingly being used to understand human diseases, due to its high similarity to the human genome and its optical transparency during embryonic stages. Once the zebrafish has fully developed, especially wild-type breeds, conventional optical imaging techniques have difficulty in imaging the internal organs and structures with sufficient resolution and penetration depth. Even with established mutant lines that remain transparent throughout their life cycle, it is still challenging for purely optical imaging modalities to visualize the organs of juvenile and adult zebrafish at a micro-scale spatial resolution. In this work, we developed a non-invasive three-dimensional photoacoustic imaging platform with an optimized illumination pattern and a cylindrical-scanning-based data collection system to image entire zebrafish with micro-scale resolutions of 80 mu m and 600 mu m in the lateral and axial directions, respectively. In addition, we employed a multispectral strategy that utilized excitation wavelengths from 690 nm to 930 nm to statistically quantify the relative optical absorption spectrum of major organs. (C) 2016 Optical Society of America

DOI10.1364/BOE.7.003543
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS SubjectBiochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS IDWOS:000385416500031
PublisherOPTICAL SOC AMER
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Citation statistics
Cited Times [WOS]:9   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorXi, Lei
Affiliation1.School of Physical Electronics, University of Electronic Science and Technology of China, Chengdu, Sichuan, China
2.Center for Information in Biomedicine, University of Electronic Science and Technology of China, Sichuan, China
3.Faculty of Health Sciences, University of Macau, Macau, China
4.These authors contributed equally to this work
Recommended Citation
GB/T 7714
Huang, Na,Gu, Heng,Qi, Weizhi,et al. Whole-body multispectral photoacoustic imaging of adult zebrafish[J]. BIOMEDICAL OPTICS EXPRESS,2016,7(9):3543-3550.
APA Huang, Na.,Gu, Heng.,Qi, Weizhi.,Zhang, Zhiwei.,Rong, Jian.,...&Xi, Lei.(2016).Whole-body multispectral photoacoustic imaging of adult zebrafish.BIOMEDICAL OPTICS EXPRESS,7(9),3543-3550.
MLA Huang, Na,et al."Whole-body multispectral photoacoustic imaging of adult zebrafish".BIOMEDICAL OPTICS EXPRESS 7.9(2016):3543-3550.
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