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Decoding glycome of Astragalus membranaceus based on pressurized liquid extraction, microwave-assisted hydrolysis and chromatographic analysis
Lv, G. P1; Hu, D. J1; Cheong, K. L1; Li, Z. Y2; Qing, X. M2; Zhao, J1; Li, S. P1
2015
Source PublicationJ. Chromatogr. A
ISSN0021-9673
Volume1409Pages:19-29
Abstract

Carbohydrates in herbs are a relatively untapped source of new drugs and health beneficial ingredients. Their analysis has been developed as a novel aspect in quality control and herbal glycomics. In this study, glycome of Astragalus membranaceus was decoded based on optimized pressurized liquid extraction (PLE), microwave-assisted acidic hydrolysis (MAAH) and comprehensive chromatographic approaches. Twelve saccharides including sucrose, galacturonic acid, mannitol, fructose, rhamnose, ribose, arabinose, fucose, xylose, mannose, glucose and galactose were quantitatively analyzed by GC–MS and HPLC–CAD (charged aerosol detectors). Different columns, including Prevail Carbohydrate ES, XBridge Amide and CARBOSep CHO-820 CA for HPLC–CAD analysis, were compared for evaluation of oligosaccharides. The polysaccharides in water extract of Astragalus membranaceus were characterized by high performance size exclusive chromatography (HPSEC) combined with multiple angle light scattering detection (MALSD) and refractive index detection (RID). The results showed that A. membranaceus contained more than 108.5 mg g−1 free sucrose and small amounts of glucose 9.6–26.0 mg g−1 and fructose 8.7–22.9 mg g−1. While its polymeric carbohydrates were composed of glucose 71.0–162.3 mg g−1, galacturonic acid 52.0–113.4 mg g−1, arabinose 22.8–54.4 mg g−1 and small amounts of galactose, rhamnose, xylose and mannose. CARBOSep CHO-820 CA showed its potential in simultaneously analyzing oligosaccharides and uronic acid, especially only the environment-friendly water mobile phase was used. HPSEC–MALSD–RID showed that there were three different molecular weight distributions of polysaccharides in A. membranaceus and the average molecular weight were 21901.1, 2038.5, and 353.4 kDa. Hierarchical clustering analysis and principal component analysis demonstrated that A. membranaceus from different regions showed variations both in free and polymeric carbohydrates, which indicated that carbohydrates should be evaluated for the proper quality control of A. membranaceus. Rha, Ara, Xyl, Man and Gal were found to be the main elements for quality evaluation of polymeric carbohydrates in A. membranaceus by factor analysis. The strategy for decoding the glycome based on chromatographic approaches including GC–MS, HPLC–CAD and HPSEC–MALSD–RID after pressurized liquid extraction and microwave-assisted hydrolysis could be applied for carbohydrates profiling in herbs and beneficial for their quality control.

KeywordCarbohydrates Gc–ms Hplc–cad Hpsec–malsd–rid Polysaccharides Quality Control
DOIhttps://doi.org/10.1016/j.chroma.2015.07.058
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000359882600003
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Cited Times [WOS]:13   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China
2.Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan, China
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Lv, G. P,Hu, D. J,Cheong, K. L,et al. Decoding glycome of Astragalus membranaceus based on pressurized liquid extraction, microwave-assisted hydrolysis and chromatographic analysis[J]. J. Chromatogr. A,2015,1409:19-29.
APA Lv, G. P.,Hu, D. J.,Cheong, K. L.,Li, Z. Y.,Qing, X. M.,...&Li, S. P.(2015).Decoding glycome of Astragalus membranaceus based on pressurized liquid extraction, microwave-assisted hydrolysis and chromatographic analysis.J. Chromatogr. A,1409,19-29.
MLA Lv, G. P,et al."Decoding glycome of Astragalus membranaceus based on pressurized liquid extraction, microwave-assisted hydrolysis and chromatographic analysis".J. Chromatogr. A 1409(2015):19-29.
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