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Fast identification of anticancer constituents in Forsythiae Fructus based on metabolomics approaches
Bao, Jiaolin1; Ding, Ren-Bo2; Jia, Xuejing1; Liang, Yeer1; Liu, Fang1; Wang, Kai1; Zhang, Chao1,3; Li, Peng1; Wang, Yitao1; Wan, Jian-Bo1; He, Chengwei1
2018-05-30
Source PublicationJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN0731-7085
Volume154Pages:312-320
Abstract

An herb commonly contains hundreds of constituents. Identification of bioactive compound(s) in each herb using conventional approaches is usually inefficient and eco-unfriendly. In this study, we aimed to fast identify anticancer compounds in Forsythiae Fructus using UPLC/MS-based metabolomics analysis. We firstly fractionated Forsythiae Fructus crude extracts with organic solvents of different polarity, then the chemical profile of each fraction was analyzed by UPLC/Q-TOF/MS, and the anticancer activity profiles of all fractions were determined by MIT assay. Next, orthogonal projections to latent structures discriminant analysis (OPLS-DA) was applied to discriminate fractions with different anticancer activity to determine the compound(s) that contributes most to the anticancer activity. Betulinic acid was then identified to be the most potent anticancer compound in Forsythiae Fructus. Its predicted anticancer activity was confirmed by MIT assay. Taken together, our results demonstrated that the present integrated metabolomics strategy could be used for fast identification of anticancer compound(s) in herb extracts or other complex mixtures of chemicals. (C) 2018 Elsevier B.V. All rights reserved.

KeywordForsythiae Fructus Anticancer Metabolomics Multivariate Data Analysis B16-f10 Melanoma
DOIhttp://doi.org/10.1016/j.jpba.2018.03.020
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS SubjectChemistry, Analytical ; Pharmacology & Pharmacy
WOS IDWOS:000431285100036
PublisherELSEVIER SCIENCE BV
The Source to ArticleWOS
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Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorHe, Chengwei
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, 999078, China
2.Faculty of Health Sciences, University of Macau, Macao, 999078, China
3.School of Life Sciences, Beijing University of Chinese Medicine, Beijing, 100029, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Bao, Jiaolin,Ding, Ren-Bo,Jia, Xuejing,et al. Fast identification of anticancer constituents in Forsythiae Fructus based on metabolomics approaches[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2018,154:312-320.
APA Bao, Jiaolin.,Ding, Ren-Bo.,Jia, Xuejing.,Liang, Yeer.,Liu, Fang.,...&He, Chengwei.(2018).Fast identification of anticancer constituents in Forsythiae Fructus based on metabolomics approaches.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,154,312-320.
MLA Bao, Jiaolin,et al."Fast identification of anticancer constituents in Forsythiae Fructus based on metabolomics approaches".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 154(2018):312-320.
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