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MEK1/2 regulates microtubule organization, spindle pole tethering and asymmetric division during mouse oocyte meiotic maturation
Yu L.-Z.2; Xiong B.2; Gao W.-X.5; Wang C.-M.2; Zhong Z.-S.2; Huo L.-J.2; Wang Q.2; Hou Y.2; Liu K.7; Liu X.J.6; Schatten H.1; Chen D.-Y.2; Sun Q.-Y.2
2007-02-01
Source PublicationCell Cycle
ISSN15514005 15384101
Volume6Issue:3Pages:330-338
AbstractIt is well known that MAPK plays pivotal roles in oocyte maturation, but the function of MEK (MAPK kinase) remains unknown. We have studied the expression, subcellular localization and functional roles of MEK during meiotic maturation of mouse oocytes. Firstly, we found that MEK1/2 phoshorylation (p-MEK1/2, indicative of MEK activation) was low in GV (germinal vesicle) stage, increased 2h after GVBD (germinal vesicle breakdown), and reached the maximum at metaphase II. Secondly, we found that P-MEK1/2 was restricted in the GV prior to GVBD. In prometaphase I and metaphase I, P-MEK1/2 was mainly associated with the spindle, especially with the spindle poles. At anaphase I and telophase I, p-MEK1/2 became diffusely distributed in the region between the separating chromosomes, and then became associated with the midbody. The association of p-MEK1/2 with spindle poles was further confirmed by its colocalization with the centrosomal proteins, γ-tubulin and NuMA. Thirdly, we have investigated the possible functional role of MEK1/2 activation by intravenous administration and intrabursal injection of a specific MEK inhibitor, U0126, and by microinjection of MEK siRNA into oocytes. All these manipulations cause disorganized spindle poles and spindle structure, misaligned chromosomes and larger than normal polar bodies. Our results suggest that MEK1/2 may function as a centrosomal protein and may have roles in microtubule organization, spindle pole tethering and asymmetric division during mouse oocyte maturation. ©2007 Landes Bioscience.
KeywordCentrosomal proteins Meiotic maturation MEK Mouse oocytes Spindle
DOI10.4161/cc.6.3.3805
URLView the original
Language英語
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Cited Times [WOS]:64   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.University of Missouri-Columbia
2.Institute of Zoology Chinese Academy of Sciences
3.Stowers Institute for Medical Research
4.Chinese Academy of Sciences
5.Peking University
6.Ottawa Hospital Research Institute
7.Umea Universitet
Recommended Citation
GB/T 7714
Yu L.-Z.,Xiong B.,Gao W.-X.,et al. MEK1/2 regulates microtubule organization, spindle pole tethering and asymmetric division during mouse oocyte meiotic maturation[J]. Cell Cycle,2007,6(3):330-338.
APA Yu L.-Z..,Xiong B..,Gao W.-X..,Wang C.-M..,Zhong Z.-S..,...&Sun Q.-Y..(2007).MEK1/2 regulates microtubule organization, spindle pole tethering and asymmetric division during mouse oocyte meiotic maturation.Cell Cycle,6(3),330-338.
MLA Yu L.-Z.,et al."MEK1/2 regulates microtubule organization, spindle pole tethering and asymmetric division during mouse oocyte meiotic maturation".Cell Cycle 6.3(2007):330-338.
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