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Screening and identification of four PAHs-biodegrading strains
Wang C.1; Li D.2; Liu S.1
2007-08-25
Source PublicationChinese Journal of Applied and Environmental Biology
ISSN1006687X
Volume13Issue:4Pages:546-550
AbstractMicrobes play an important role in bio-remediation technologies, and it is a basic study to find out effectively functional strains for environmental bio-remediation. In this paper four PAHs-biodegrading strains were screened with a selective medium of PAHs from oily wastewater and oil-contaminated soil. The four strains were identified by their morphological, physiological and biochemical characteristics and gene analyses, and they were named as 3-28, NF, EW and EY. The 16S rDNA of the strains was amplified by PCR, indicating that they possessed 581, 582, 1209, 1 230 bp, respectively, which were compared with those available in the GenBank databases. The results showed that the 16S rDNA sequence of 3-28 strain was mostly similar to that of Microbacterium esteraromaticum, NF similar to Cellulosimicrobium cellulans, EW to Chelatococcus asacharovorans and EY to Sphingopyxis terrae, and their sequence similarities were 100%, 97.8%, 98.2% and 99.0%, respectively. The phylogenetic trees of the four strains were derived with DNAMAN and analyzed with Bootstraping. The results demonstrate that the 3-28, NF, EW and EY strains belonged to Microbacterium sp., Cellulosimicrobium sp., Chelatococcus sp. and Sphingopyxis sp., respectively. Fig 3, Tab 1, Ref 22.
Keyword16S rDNA Biodegradation Polycyclic aromatic hydrocarbons (PAHs) Sequence similarity
URLView the original
Language英語
Fulltext Access
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Sichuan University
2.Chengdu Institute of Biology Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang C.,Li D.,Liu S.. Screening and identification of four PAHs-biodegrading strains[J]. Chinese Journal of Applied and Environmental Biology,2007,13(4):546-550.
APA Wang C.,Li D.,&Liu S..(2007).Screening and identification of four PAHs-biodegrading strains.Chinese Journal of Applied and Environmental Biology,13(4),546-550.
MLA Wang C.,et al."Screening and identification of four PAHs-biodegrading strains".Chinese Journal of Applied and Environmental Biology 13.4(2007):546-550.
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