UM
BcLTP, a novel lipid transfer protein in Brassica chinensis, may secrete and combine extracellular CaM
Wang C.2; Xie W.1; Chi F.1; Hu W.1; Mao G.2; Sun D.2; Li C.1; Sun Y.2
2008
Source PublicationPlant Cell Reports
ISSN07217714
Volume27Issue:1Pages:159-169
AbstractLipid transfer proteins in plants are believed to be involved in many processes of cell physiology and development. In this work, a full-length cDNA encoding a novel lipid transfer protein, designated BcLTP was isolated from Brassica chinensis. At least two copies of BcLTP are present in whole genome of B. chinensis, and its transcripts preferably accumulate in second-year organs, implying its role in reproductive growth stage. The 118 amino acid sequence deduced from a 354 bp open reading frame (ORF) shares common features with other members of plants LTPs family. A putative signal peptide at the N terminus was tested for secretion function by the yeast signal sequence trap (YSST) system, and further confirmed by vesicular and extracellular localization of YFP fusion protein. A highly conserved CaM binding site at C terminus was found and the binding properties with two representative CaM isoforms, one is convergent AtCaM2, one is divergent SCaM5, were determined by gel overlay. We found that convergent AtCaM2 prefer high concentration of Ca for binding BcLTP, while SCaM5 does not depend on Ca concentration too much for binding BcLTP. The lipid binding feature of BcLTP was demonstrated using florescence-marked 1-pyrenedodecanoic acid, which can be enhanced by AtCaM2 in Ca dependent manner and by SCaM5 in either presence or absence of Ca. The collected data suggest that BcLTP may secrete and combine extracellular CaM isoforms, which in turn, facilitate lipid binding of BcLTP via Ca mediated signaling. © 2007 Springer-Verlag.
KeywordBrassica chinensis CaM isoforms Extracellular space Lipid transfer protein
DOI10.1007/s00299-007-0434-4
URLView the original
Language英語
Fulltext Access
Citation statistics
Cited Times [WOS]:11   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Nankai University
2.Hebei Normal University
Recommended Citation
GB/T 7714
Wang C.,Xie W.,Chi F.,et al. BcLTP, a novel lipid transfer protein in Brassica chinensis, may secrete and combine extracellular CaM[J]. Plant Cell Reports,2008,27(1):159-169.
APA Wang C..,Xie W..,Chi F..,Hu W..,Mao G..,...&Sun Y..(2008).BcLTP, a novel lipid transfer protein in Brassica chinensis, may secrete and combine extracellular CaM.Plant Cell Reports,27(1),159-169.
MLA Wang C.,et al."BcLTP, a novel lipid transfer protein in Brassica chinensis, may secrete and combine extracellular CaM".Plant Cell Reports 27.1(2008):159-169.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang C.]'s Articles
[Xie W.]'s Articles
[Chi F.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang C.]'s Articles
[Xie W.]'s Articles
[Chi F.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang C.]'s Articles
[Xie W.]'s Articles
[Chi F.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.