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Enhancing cell affinity of nonadhesive hydrogel substrate: The role of silica hybridization
Wang C.; Bai J.; Gong Y.; Zhang F.; Shen J.; Wang D.-A.
2008-09-01
Source PublicationBiotechnology Progress
ISSN87567938
Volume24Issue:5Pages:1142-1146
Abstract

The aim of this study is to elucidate the functional mechanism of nanosilica-induced cell affinity for the normally attachment-fouling hydrogel substrates. Investigations were conducted in the following three major aspects: (1) environmental protein adsorption before cell contacts, which determines the presence of adhesive ligands; (2) integrin expression profile, which reflects the cell responses via surface receptor turning-over; and (3) vinculin activation and F-actin assembly, which sketches the induced cytoskeletal organization posing for focal adhesion. The results indicate that the hybridized nanosilica additives are capable of arresting adhesive proteins from the environment. As binding ligands, such immobilized proteins activate α5β1-specific pathway for cell focal adhesion, but fail in invoking β3-participated signaling cascade. This partial activation enables modest amount of high-quality cell adhesion on the modified substrate, by which a conservative cell affinity is established on hydrogel matrices. © 2008 American Institute of Chemical Engineers.

KeywordCell Affinity Focal Adhesion Fumed Silica Hydrogel Integrins
DOI10.1002/btpr.52
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Food Science & Technology
WOS SubjectBiotechnology & Applied Microbiology ; Food Science & Technology
WOS IDWOS:000260738900017
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Cited Times [WOS]:13   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
AffiliationNanyang Technological University
Recommended Citation
GB/T 7714
Wang C.,Bai J.,Gong Y.,et al. Enhancing cell affinity of nonadhesive hydrogel substrate: The role of silica hybridization[J]. Biotechnology Progress,2008,24(5):1142-1146.
APA Wang C.,Bai J.,Gong Y.,Zhang F.,Shen J.,&Wang D.-A..(2008).Enhancing cell affinity of nonadhesive hydrogel substrate: The role of silica hybridization.Biotechnology Progress,24(5),1142-1146.
MLA Wang C.,et al."Enhancing cell affinity of nonadhesive hydrogel substrate: The role of silica hybridization".Biotechnology Progress 24.5(2008):1142-1146.
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