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An improved injectable polysaccharide hydrogel: Modified gellan gum for long-term cartilage regeneration in vitro
Gong Y.; Wang C.; Lai R.C.; Su K.; Zhang F.; Wang D.-A.
2009-03-31
Source PublicationJournal of Materials Chemistry
ISSN09599428 13645501
Volume19Issue:14Pages:1968-1977
Abstract

Polysaccharide-based hydrogels have been proven promising in tissue engineering applications due to their good biocompatibility, controllable properties and abundance, and are therefore in great demand as therapeutic cell vehicles. Gellan gum, a natural polysaccharide and FDA-approved food additive, has been well exploited in food and pharmaceutical industries. For tissue engineering purposes, however, gellan-based hydrogels need to be modified in order to meet the requirement of encapsulating living cells while maintaining their injectability, because the gelling point of this temperature-dependent gel is too high (above 42 °C). This study started from chemically scissoring (via oxidative cleavage) the gellan backbones to optimize the gelation temperature for injection as a result of down-regulating their molecular size. Chondrocytes were then seeded into the modified gellan gels, the cytocompatibility and the capability to promote in vitro tissue regeneration of which were evaluated. Notably, chondrocytic constructs based on modified gellan gel were kinetically monitored for 150 days in comparison with those based on agarose gel, showing superiority for long-term cartilaginous development in terms of many aspects such as cell proliferation and specific matrix formation. Biochemical analysis, histological staining, and immunofluorescent observation indicate that the modified gellan is able to retain the chondrocytes viability, enhance the extracellular matrix (ECM) secretion and maintain normal phenotype, which demonstrates that gellan is a potential and promising injectable vehicle for therapeutic chondrocyte delivery. © The Royal Society of Chemistry 2009.

DOI10.1039/b818090c
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Materials Science
WOS SubjectChemistry, Physical ; Materials Science, Multidisciplinary
WOS IDWOS:000264524200006
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Cited Times [WOS]:70   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
AffiliationNanyang Technological University
Recommended Citation
GB/T 7714
Gong Y.,Wang C.,Lai R.C.,et al. An improved injectable polysaccharide hydrogel: Modified gellan gum for long-term cartilage regeneration in vitro[J]. Journal of Materials Chemistry,2009,19(14):1968-1977.
APA Gong Y.,Wang C.,Lai R.C.,Su K.,Zhang F.,&Wang D.-A..(2009).An improved injectable polysaccharide hydrogel: Modified gellan gum for long-term cartilage regeneration in vitro.Journal of Materials Chemistry,19(14),1968-1977.
MLA Gong Y.,et al."An improved injectable polysaccharide hydrogel: Modified gellan gum for long-term cartilage regeneration in vitro".Journal of Materials Chemistry 19.14(2009):1968-1977.
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