UM  > 個人在本單位外知識產出
Mutations in the cytoplasmic domain of the 275 kd mannose 6-phosphate receptor differentially alter lysosomal enzyme sorting and endocytosis
Lobel P.1; Fujimoto K.1; Ye R.D.1; Griffiths G.1; Kornfeld S.1
1989-06-02
Source PublicationCell
ISSN00928674
Volume57Issue:5Pages:787-796
AbstractThe cation-independent mannose 6-phosphate receptor (CI-MPR) sorts newly synthesized lysosomal enzymes in the Golgi and endocytoses extracellular lysosomal enzymes. To determine the role of the 163 amino acid cytoplasmic domain of the CI-MPR in these functions, receptor-deficient mouse L cells were transfected with normal bovine CI-MPR cDNA or cDNAs mutated in the cytoplasmic domain. The normal CI-MPR functioned in sorting and endocytosis. Mutant receptors with 40 and 89 residues deleted from the carboxyl terminus of the cytoplasmic tail functioned normally in endocytosis, but were partially impaired in sorting. Mutant receptors with larger deletions leaving only 7 and 20 residues of the cytoplasmic tail were defective in endocytosis and sorting. A mutant receptor containing alanine instead of tyrosine residues at positions 24 and 26 was defective in endocytosis, and partially impaired in sorting. Receptors deficient in endocytosis accumulated at the cell surface. These results indicate that the cytoplasmic domain of the CI-MPR contains different signals for rapid endocytosis and efficient lysosomal enzyme sorting. © 1989.
DOI10.1016/0092-8674(89)90793-9
URLView the original
Language英語
Citation statistics
Cited Times [WOS]:252   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionPersonal research not belonging to the institution
Affiliation1.Washington University School of Medicine in St. Louis
2.European Molecular Biology Laboratory Heidelberg
Recommended Citation
GB/T 7714
Lobel P.,Fujimoto K.,Ye R.D.,et al. Mutations in the cytoplasmic domain of the 275 kd mannose 6-phosphate receptor differentially alter lysosomal enzyme sorting and endocytosis[J]. Cell,1989,57(5):787-796.
APA Lobel P.,Fujimoto K.,Ye R.D.,Griffiths G.,&Kornfeld S..(1989).Mutations in the cytoplasmic domain of the 275 kd mannose 6-phosphate receptor differentially alter lysosomal enzyme sorting and endocytosis.Cell,57(5),787-796.
MLA Lobel P.,et al."Mutations in the cytoplasmic domain of the 275 kd mannose 6-phosphate receptor differentially alter lysosomal enzyme sorting and endocytosis".Cell 57.5(1989):787-796.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Lobel P.]'s Articles
[Fujimoto K.]'s Articles
[Ye R.D.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Lobel P.]'s Articles
[Fujimoto K.]'s Articles
[Ye R.D.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Lobel P.]'s Articles
[Fujimoto K.]'s Articles
[Ye R.D.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.